TIP, Genel Cerrahi Anabilim Dalı, Makale Koleksiyonuhttp://hdl.handle.net/11616/117312024-03-19T14:03:36Z2024-03-19T14:03:36ZQuercetin dose affects the fate of hepatic ischemia and reperfusion injury in rats: An experimental researchUylas, Mustafa Ufukhttp://hdl.handle.net/11616/129222019-07-26T00:02:18Z2018-01-01T00:00:00ZQuercetin dose affects the fate of hepatic ischemia and reperfusion injury in rats: An experimental research
Uylas, Mustafa Ufuk
BACKGROUND: Quercetin found in fruits and vegetables has an antioxidative effect. We aimed to investigate the protective effects of quercetin according to different doses on hepatic and ischemia-reperfusion (I/R) injury.
METHODS: Fifty mature male Sprague-Dawley rats were randomly divided into five groups (n = 10 for each). All the animal groups underwent laparotomy. Group 1 rats served as a sham-operated group. Groups 2-5 underwent 1 h hepatic ischemia and were followed by 2 h reperfusion. Group 3-5 animals received an additional intraperitoneal dose of 25, 50 or 100 mg/kg quercetin respectively before I/R operation. Blood samples were collected for determining serum aspartate transaminase (AST), alanine transaminase (ALT) and malondialdehyde (MDA) levels. Also, liver tissue samples were taken for measuring of liver MDA concentration and for histopathology assessment.
RESULTS: The highest levels of biochemical parameters were observed in group 2. In quercetin-treated groups, serum AST, ALT, MDA levels, and tissue MDA concentration were decreased as inversely with increasing quercetin dose. Microscopic evaluation revealed that most conspicuous histological improvement was observed in 50 mg/kg quercetin co-treated rats. 25 and 100 mg/kg quercetin co-treatment could not protect completely against hepatic I/R injury.
CONCLUSION: Quercetin can be effective in preventing of hepatic I/R injury when the correct dose was used.
2018-01-01T00:00:00ZInvestigation of the effect of milrinone on renal damage in an experimental non-heart beating donormodelBatcıoğlu, KadirUyumlu, Burçin A.Karadağ, Mehmethttp://hdl.handle.net/11616/127292019-07-19T00:00:45Z2018-01-01T00:00:00ZInvestigation of the effect of milrinone on renal damage in an experimental non-heart beating donormodel
Batcıoğlu, Kadir; Uyumlu, Burçin A.; Karadağ, Mehmet
Purpose: In our study, it was aimed to investigate the preventive effect of milrinone on renal damage in experimental controlled non-heart-beating donors (NHBDs) model. Materials and Methods: Sixteen rats randomly divided into 2 groups, 8 rats in each were used. Group 1 was control, group 2 was milrinone group. Group 1 rats received 1.25ml 0.09% NaCl intraperitoneally equivalent to the milrinone diluted volume. Group 2 rats were administered intraperitoneally with 0.5mg/kg of milrinone 2hours before cardiac arrest. After the cardiac arrest, left nephrectomy was applied to the rats. Malondialdehyde, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) activities, Caspase-3 (apoptotic index) and histopathological evaluation were performed in the tissues. Results: In the milrinone group, the total injury score was significantly lower relative to the control group (p = 0.001). Caspase-3 staining was moderately strong in the control group but weaker in the milrinone group. Apoptotic index was significantly lower in the milrinone group compared to the control group (p = 0.001). In comparison between groups, SOD and GPx in the milrinone group was significantly higher than the control group (p = 0.008, p = 0.006). Conclusions: Milrinone has been shown to be effective in the prevention of tissue damage due to oxidative stress and inflammatory process in the renal of warm ischemia in the experimental NHBDs model and in protecting the renal. Milrinone increases antioxidant activity while reducing apoptosis. Systemic administration of milrinone prior to cardiac arrest may be beneficial. Administration of milrinone to the recipient in the perioperative period may contribute to donor function.
2018-01-01T00:00:00ZAn intrahepatic portal vein aneurysm presenting with esophageal variceal bleeding in a pediatricpatient: a rare clinical entityGüngör, ŞükrüVarol, Fatma ilknurKutlu, RamazanYılmaz, SezaiSelimoğlu, Mukadder Ayşehttp://hdl.handle.net/11616/122942019-07-05T00:02:24Z2018-01-01T00:00:00ZAn intrahepatic portal vein aneurysm presenting with esophageal variceal bleeding in a pediatricpatient: a rare clinical entity
Güngör, Şükrü; Varol, Fatma ilknur; Kutlu, Ramazan; Yılmaz, Sezai; Selimoğlu, Mukadder Ayşe
2018-01-01T00:00:00ZA surgical technique for secondary repair of obstetric anal sphincter injuries; Sphinctero-vagino-perineoplastyŞimşek, ArifeAteş, MustafaDirican, AbuzerÖzgör, Dinçerhttp://hdl.handle.net/11616/114352019-07-05T12:58:59Z2018-01-01T00:00:00ZA surgical technique for secondary repair of obstetric anal sphincter injuries; Sphinctero-vagino-perineoplasty
Şimşek, Arife; Ateş, Mustafa; Dirican, Abuzer; Özgör, Dinçer
Objective: This study was conducted to present the preliminary results of seven patients treated with sphinctero-vagino-perineoplasty for secondary repair of obstetric anal sphincter injuries.
Materials and Methods: This retrospective study was conducted on the records of seven patients who underwent secondary repair of obstetric anal sphincter injuries at the colorectal surgery unit of a tertiary care center between February 2015 and December 2017.
Results: All patients with solid stool incontinence were fully recovered at postoperative month 3. The Wexner incontinence score was significantly improved (decreased from 14.12 [range: 8-20] to 2.28 [range: 1-4]). The complication rate was 85.7% (wound infection, abscess, hematoma, detachment).
Conclusion: Combined repair of anal sphinchters, perineal body, superficial transverse perineal muscles, and bulbospongious muscles, which contribute to anal continence, may improve surgical outcomes in patients with obstetric anal sphincter injuries.
2018-01-01T00:00:00Z