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Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B- and siPDGFR-beta-Containing Chitosan Nanoplexes

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dc.contributor.author Salva, Emine
dc.contributor.author Turan, Suna Ozbas
dc.contributor.author Akbuga, Julide
dc.date.accessioned 2019-08-09T11:32:33Z
dc.date.available 2019-08-09T11:32:33Z
dc.date.issued 2017
dc.identifier.citation Salva, E. Turan, SO . Akbuga, J . (2017). Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B- and siPDGFR-beta-Containing Chitosan Nanoplexes.Cilt:18. Sayı:4. 1031-1042 ss. tr_TR
dc.identifier.uri http://hdl.handle.net/11616/13370
dc.description.abstract Mesangioproliferative glomerulonephritis is a disease that has a high incidence in humans. In this disease, the proliferation of glomerular mesangial cells and the production of extracellular matrix are important. In recent years, the RNAi technology has been widely used in the treatment of various diseases due to its capability to inhibit the gene expression with high specificity and targeting. The objective of this study was to decrease mesangial cell proliferation by knocking down PDGF-B and its receptor, PDGFR-beta. To be able to use small interfering RNAs (siRNAs) in the treatment of this disease successfully, it is necessary to develop appropriate delivery systems. Chitosan, which is a biopolymer, is used as a siRNA delivery system in kidney drug targeting. In order to deliver siRNA molecules targeted at PDGF-B and PDGFR-beta, chitosan/siRNA nanoplexes were prepared. The in vitro characterization, transfection studies, and knockdown efficiencies were studied in immortalized and primary rat mesangial cells. In addition, the effects of chitosan nanoplexes on mesangial cell proliferation and migration were investigated. After in vitro transfection, the PDGF-B and PDGFR-beta gene silencing efficiencies of PDGF-B and PDGFR-beta targeting siRNA-containing chitosan nanoplexes were 74 and 71% in immortalized rat mesangial cells and 66 and 62% in primary rat mesangial cells, respectively. siPDGF-B- and siPDGFR-beta-containing nanoplexes indicated a significant decrease in mesangial cell migration and proliferation. These results suggested that mesangial cell proliferation may be inhibited by silencing of the PDGF-B signaling pathway. Gene silencing approaches with chitosan-based gene delivery systems have promise for the efficient treatment of renal disease. tr_TR
dc.language.iso eng tr_TR
dc.publisher Sprınger, 233 sprıng st, new york, ny 10013 usa tr_TR
dc.relation.isversionof 10.1208/s12249-016-0687-8 tr_TR
dc.rights info:eu-repo/semantics/openAccess tr_TR
dc.subject Molecular-weıght chıtosan tr_TR
dc.subject endothelıal growth-factor tr_TR
dc.subject n-succınyl-chıtosan tr_TR
dc.subject sırna delıvery tr_TR
dc.subject ın-vıvo tr_TR
dc.subject nanopartıcle formulatıon tr_TR
dc.subject renal-dısease tr_TR
dc.subject drug carrıer tr_TR
dc.subject pdgf tr_TR
dc.subject rat tr_TR
dc.title Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B- and siPDGFR-beta-Containing Chitosan Nanoplexes tr_TR
dc.type article tr_TR
dc.relation.journal Aaps pharmscıtech tr_TR
dc.contributor.department İnönü Üniversitesi tr_TR
dc.identifier.volume 18 tr_TR
dc.identifier.issue 4 tr_TR
dc.identifier.startpage 1031 tr_TR
dc.identifier.endpage 1042 tr_TR

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