DSpace Repository

In Vitro PDGF-B Gene Silencing Studies and In Vivo Delivery of siRNA to the Rat Kidney Using Chitosan/siRNA Nanoplexes

Show simple item record

dc.contributor.author Salva, Emine
dc.contributor.author Ozbas Turan, Suna
dc.contributor.author Alan, Saadet
dc.contributor.author Akbuga, Julide
dc.date.accessioned 2019-09-24T11:37:31Z
dc.date.available 2019-09-24T11:37:31Z
dc.date.issued 2016
dc.identifier.citation Salva, E . Ozbas Turan, S . Alan, S. Akbuga, J . (2016). In Vitro PDGF-B Gene Silencing Studies and In Vivo Delivery of siRNA to the Rat Kidney Using Chitosan/siRNA Nanoplexes. Cilt:20. Sayı:3. 263- 268 ss. tr_TR
dc.identifier.uri http://hdl.handle.net/11616/14249
dc.description.abstract The targeting of specific genes responsible from onset and progression of kidney diseases offer a new therapeutic strategy in the field of renal gene therapy. The altered expression of platelet derived growth factor (PDGF) is an important marker of renal diseases. In this study, we investigated in vitro gene silencing efficiency of chitosan nanoplexes containing PDGF-B and PDGFR-beta targeted siRNAs in the kidney cell lines including HEK-293 and MDCK and delivery to the kidney as an in vivo delivery system. As a result, PDGF-B expression was significantly inhibited by co-delivery of chitosan/siPDGF-B+siPDGFR-beta nanoplexes prepared using in the different weight ratios (10/1, 20/1 and 50/1). When 20/1 and 50/1 weight ratios of chitosan nanoplexes were i.v. injected to rats, chitosan/FITC-siPDGFB nanoplexes were reached to kidney tissue at 4 h after intravenous injection. These results suggest that delivery of siRNA using chitosan nanoplexes may be effective for the therapy of kidney diseases. tr_TR
dc.language.iso eng tr_TR
dc.publisher Marmara unıv, fac medıcıne, haydarpasa, ıstan, 34668, turkey tr_TR
dc.relation.isversionof 10.12991/mpj.20162082721 tr_TR
dc.rights info:eu-repo/semantics/openAccess tr_TR
dc.subject Small ınterferıng rna tr_TR
dc.subject growth-factor tr_TR
dc.subject nanopartıcles tr_TR
dc.subject potentıals tr_TR
dc.subject therapy tr_TR
dc.subject dısease tr_TR
dc.subject vegf tr_TR
dc.title In Vitro PDGF-B Gene Silencing Studies and In Vivo Delivery of siRNA to the Rat Kidney Using Chitosan/siRNA Nanoplexes tr_TR
dc.type article tr_TR
dc.relation.journal Marmara pharmaceutıcal journal tr_TR
dc.contributor.department İnönü Üniversitesi tr_TR
dc.identifier.volume 20 tr_TR
dc.identifier.issue 3 tr_TR
dc.identifier.startpage 263 tr_TR
dc.identifier.endpage 268 tr_TR


Files in this item

This item appears in the following Collection(s)

Show simple item record