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Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method

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dc.contributor.author Kaynak, Mustafa Sinan
dc.contributor.author Büyüktuncel, Ebru
dc.contributor.author Çağlar, Hatice
dc.contributor.author Şahin, Selma
dc.date.accessioned 2020-03-10T10:56:49Z
dc.date.available 2020-03-10T10:56:49Z
dc.date.issued 2015
dc.identifier.citation Kaynak, M. S., Büyüktuncel, E., Çaglar, H., Çaglar, H., Şahin, S. (2015). Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method. İnönü Üniversitesi, Malatya. tr_TR
dc.identifier.issn 1596-5996
dc.identifier.uri http://hdl.handle.net/11616/15354
dc.description.abstract Purpose: To develop a simple and rapid reversed-phase high performance liquid chromatographic (HPLC) method with UV detection for the simultaneous determination of diclofenac, metoprolol tartrate, phenol red and propyl paraben in intestinal segments. Methods: The mobile phase consisted of 55% methanol, 45% of 12.5 mM potassium dihydrogen phosphate (KH2PO4) aqueous solution, adjusted to pH 7.0 using 0.2 N sodium hydroxide (NaOH) solution, and to which 0.3% (v/v) triethylamine was added. Analysis was run at a flow rate of 1.0 mL/min with a 12 min total run time at ambient temperature. The developed method was successfully applied to determination of the analytes in samples obtained from in situ single pass intestinal perfusion (SPIP) studies. Results: The calibration curves were linear for all compounds (r > 0.999) with a limit of detection (LOD) of 0.005, 0.1, 0.075 mu g/mL, and limit of quantification of 0.1, 0.3, 0.2 mu g/mL for metoprolol tartrate, phenol red and diclofenac respectively. The coefficient of variation for intra-day and inter-day precision was < 5% and accuracy was between 98 and 102%. Based on SPIP and HPLC studies, the estimated mean permeability in jejunum, ileum and colon was 0.319 +/- 0.184, 0.639 +/- 0.241 and 0.84 3 +/- 0.517 x 10(-4) cm/sec, respectively, for metoprolol tartrate while the corresponding permeability values were 1.585 +/- 0.729, 1.154 +/- 0.433 and 1.775 +/- 1.576 x 10(-4) cm/sec for diclofenac. Conclusion: The findings indicate that diclofenac is a highly permeable compound and its absorption occurs throughout the intestinal tract. Furthermore, the developed method is suitable for the analysis of diclofenac and metoprolol in intestinal regions. tr_TR
dc.language.iso eng tr_TR
dc.publisher PHARMACOTHERAPY GROUP tr_TR
dc.relation.isversionof 10.4314/tjpr.v14i1.23 tr_TR
dc.rights info:eu-repo/semantics/openAccess tr_TR
dc.subject Biopharmaceutics classification system tr_TR
dc.subject Diclofenac tr_TR
dc.subject Metoprolol tartrate tr_TR
dc.subject Segmental permeability tr_TR
dc.subject Intestinal absorption tr_TR
dc.subject Validation tr_TR
dc.title Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method tr_TR
dc.type article tr_TR
dc.relation.journal TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH tr_TR
dc.contributor.department İnönü Üniversitesi tr_TR
dc.contributor.authorID D-9453-2019 tr_TR
dc.contributor.authorID http://orcid.org/0000-0003-2917-2407 tr_TR
dc.contributor.authorID http://orcid.org/0000-0001-7269-6594 tr_TR
dc.identifier.volume 14 tr_TR
dc.identifier.issue 1 tr_TR
dc.identifier.startpage 163 tr_TR
dc.identifier.endpage 170 tr_TR


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