Effects of ozone application on fissure sealants prepared different surface etching modalities: An in vitro study

Abstract

Aim: The aim of this study was to compare the microleakage levels of different surface treatment of fissure sealants. Material and Methods: A total of 60 standardized non-erupted third molar teeth were divided into 5 groups (n=12) according to the pre-treatment method; Group 1 (G1): Clearfil Universal Bond Quick (self-etching) + ozone +fissure sealant (FS), Group 2 (G2): Clearfil Universal Bond Quick (self-etching) +FS, Group 3(G3): Clearfil Universal Bond Quick (etch-and-rinse) +ozone +FS, Group 4 (G4): Clearfil Universal Bond Quick (etch-and-rinse) +FS, Group 5 (G5): phosphoric acid etching +FS (control). Following surface pre-treatments, fissures of all teeth were sealed. Then, all specimens were subjected to thermal cycles (5000 times of 5 degrees C/55 degrees C for 1 minute each). All surfaces of the teeth were covered with nail polish twice up to a distance of 1mm to fissure sealant. All teeth were immersed in a 0.5% basic fuchsine dye for 24 hours. To examine microleakage, teeth separated into 2 halves bucco-lingually with saw. Microleakage scores were evaluated under stereomicroscope with a magnification of x40. The scores were statistically analyzed with Kruskal-Wallis and Mann Whitney U tests. Results: G2, G3 and G4 showed statistically significant differences compared to control group, except G1. Although G2 showed highest microleakage level, G4 sowed lowest microleakage level G5 Ozone led to decreased microleakage in images in groups 3, 5 and 6. Conclusions: The fissure sealing ability of with the universal adhesive in etch-and-rinse or self-etch modes was similar to that of conventional acid etching. Ozone pretreatment favorably affected the marginal sealing ability of the tested fissure sealants.