Microorganisms attach to surfaces and interfaces and form biofilms which create a sheltered area for host cell response. Therefore, biofilms provide troubles in fields such as medicine, food, and pharmaceuticals. Inhibition of formation of biofilms through hindering of quorum sensing could be a method for the production of new generation antibiotics. In this study, four new benzimidazole type NHC precursors (1-allyl-3-benzyl-5,6-dimethylbenzimidazolium chloride, 1-allyl-3-(2,4,6-trimethylbenzyl)-5,6-dimethylbenzimidazolium chloride, 1-allyl-3-(2,3,5,6-tetramethylbenzyl)-5,6-dimethylbenzimidazolium chloride, and 1-allyl-3-(2,3,4,5,6-pentamethylbenzyl)-5,6-dimethylbenzimidazolium chloride and Ag-NHC complexes of these molecules were synthesized and characterized by elemental analysis, FT-IR spectroscopy, H-1, and C-13{H-1} NMR spectroscopy, LC-MS, and single crystal crystallography. Antimicrobial and biofilm formation inhibition activities of the molecules were evaluated. In addition, the activities of the molecules were examined in detail by molecular docking analysis. According to the results obtained, higher activity was achieved with the complex molecules when compared with the benzimidazole derivative ligands.
C1 [Ustun, Elvan] Ordu Univ, Fac Art & Sci, Dept Chem, TR-52200 Ordu, Turkey.
[Sahin, Neslihan] Cumhuriyet Univ, Fac Educ, Dept Basic Educ, TR-58040 Sivas, Turkey.
[Sahin, Neslihan; Gurbuz, Nevin; Ozdemir, Ismail] Inonu Univ, Fac Sci & Art, Dept Chem, TR-44280 Malatya, Turkey.
[Sahin, Neslihan; Gurbuz, Nevin; Ozdemir, Ismail] Inonu Univ, Catalysis Res & Applicat Ctr, TR-44280 Malatya, Turkey.
[Celik, Cem] Cumhuriyet Univ, Fac Med, Dept Med Microbiol, Sivas, Turkey.
[Tutar, Ugur] Sivas Cumhuriyet Univ, Fac Pharm, Dept Bot, TR-58140 Sivas, Turkey.
[Ozdemir, Namik] Ondokuz Mayes Univ, Fac Educ, Dept Math & Sci Educ, TR-55139 Samsun, Turkey.
[Gurbuz, Nevin; Ozdemir, Ismail] Inonu Univ, Drug Applicat & Res Ctr, TR-44280 Malatya, Turkey.