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The electrochemical synthesis of polyaniline films was carried out by potential sweep method in sulfuric acid solution at the glassy carbon electrode. The electrocatalytic behavior to DNA bases (adenine and guanine), uric acid, and dopamine of polyaniline nanocomposite sensor modified with nano-gold particles was investigated by DPV technique. By systematically examining the effects of the possible parameters affecting the electrocatalytic performance of the relevant sensor, the optimal value for the each parameter was determined. The detailed electrocatalytic studies of the optimized sensor revealed that this sensor could be used as DNA base sensor for guanine in the presence of adenine, dopamine, and uric acid. Moreover, the performance of the relevant sensor in thermally denatured fish sperm DNA was quite satisfactory. © 2016 Taylor & Francis Group, LLC. |
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