dc.contributor.author |
Atik, Ahmet Emin |
|
dc.contributor.author |
Bozdağ, Gönensin Ozan |
|
dc.contributor.author |
Akıncı, Ersin |
|
dc.contributor.author |
Kaya, Alaattin |
|
dc.contributor.author |
Koç, Ahmet |
|
dc.contributor.author |
Yalçın, Talat |
|
dc.contributor.author |
Karakaya, Hüseyin Çağlar |
|
dc.date.accessioned |
2017-06-10T11:53:16Z |
|
dc.date.available |
2017-06-10T11:53:16Z |
|
dc.date.issued |
2011 |
|
dc.identifier.citation |
Ahmet, A., Kaya, A., Akıncı, E., Yalçın, T., Koç, A., Ozan, B., Karakaya, H. Ç. (2011). Proteomic Changes During Boron Tolerance İn Barley Hordeum Vulgare And Role Of Vacuolar Proton Translocatingatpase Subunit E . Tj Bot, (135), 379–388. |
tr_TR |
dc.identifier.uri |
http://hdl.handle.net/11616/7077 |
|
dc.description |
Turk J Bot 35 (2011) 379-388 |
tr_TR |
dc.description.abstract |
Boron is an essential micronutrient for plants and animals; however, it can be toxic when present at high
concentrations. Th e purpose of this study was to understand the mechanisms of boron tolerance in the Turkish barley
(Hordeum vulgare) Anadolu cultivar. For this purpose, 2-dimensional electrophoresis (2-DE) was used to screen
diff erentially expressed proteins for both control and boron-stressed Anadolu barley genotypes. Seven proteins were
revealed by 2-DE: 1) ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo large chain), 2) TLP5, a thaumatin-like
protein, 3) PR5, a basic pathogenesis-related protein, 4) a RNase S-like protein, 5) a PSI type III chlorophyll a/b-binding
protein, 6) a light-harvesting complex I LHC I, and 7) the vacuolar proton-translocating ATPase subunit E protein.
Th ese were found to be upregulated in response to boron treatment. Even though the protein encoded by the V-ATPase
subunit E gene was overexpressed, its transcript level was downregulated by boron treatment. Heterologous expression
of the barley V-ATPase subunit E gene in yeast provided boron resistance to yeast cells. Th ese results indicated that the
V-ATPase subunit E gene was functional and conferred tolerance to toxic boron levels in yeast and might play a role in
the overall boron tolerance of barley. |
tr_TR |
dc.language.iso |
eng |
tr_TR |
dc.publisher |
Turk J Bot |
tr_TR |
dc.rights |
info:eu-repo/semantics/openAccess |
tr_TR |
dc.subject |
Boron stress |
tr_TR |
dc.subject |
Hordeum vulgare |
tr_TR |
dc.subject |
Saccharomyces cerevisiae |
tr_TR |
dc.subject |
Proteomics |
tr_TR |
dc.subject |
V-ATPase |
tr_TR |
dc.title |
Proteomic changes during boron tolerance in barley Hordeum vulgare and role of vacuolar proton translocatingATPase subunit E |
tr_TR |
dc.type |
masterThesis |
tr_TR |
dc.relation.journal |
Turk J Bot |
tr_TR |
dc.contributor.department |
İnönü Üniversitesi |
tr_TR |
dc.contributor.authorID |
130773 |
tr_TR |
dc.contributor.authorID |
130618 |
tr_TR |
dc.contributor.authorID |
110769 |
tr_TR |
dc.identifier.volume |
135 |
tr_TR |
dc.identifier.issue |
0 |
tr_TR |
dc.identifier.startpage |
379 |
tr_TR |
dc.identifier.endpage |
388 |
tr_TR |