dc.contributor.author |
Koç, Ahmet |
|
dc.contributor.author |
Mathews, Christopher K. |
|
dc.contributor.author |
Wheeler, Linda J. |
|
dc.contributor.author |
Gross, Michael K. |
|
dc.contributor.author |
Merrill, Gary F. |
|
dc.date.accessioned |
2017-06-19T08:07:54Z |
|
dc.date.available |
2017-06-19T08:07:54Z |
|
dc.date.issued |
2006 |
|
dc.identifier.citation |
KOÇ, A., Chris, M., Linda, W., Micheal, G., & Gary, M. (2006). Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase . J Biol Chem, (281(22)), 15058–0. |
tr_TR |
dc.identifier.uri |
http://www.jbc.org/content/281/22/15058.full.pdf |
|
dc.identifier.uri |
http://hdl.handle.net/11616/7103 |
|
dc.description |
J Biol Chem |
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dc.description.abstract |
Thioredoxin was initially identified by its ability to serve as an
electron donor for ribonucleotide reductase in vitro. Whether it
serves a similar function in vivo is unclear. In Saccharomyces cerevisiae,
it was previously shown that trx1 trx2 mutants lacking the
two genes for cytosolic thioredoxin have a slower growth rate
because of a longer S phase, but the basis for S phase elongation was
not identified. The hypothesis that S phase protraction was due to
inefficient dNTP synthesis was investigated by measuring dNTP
levels in asynchronous and synchronized wild-type and trx1 trx2
yeast. In contrast to wild-type cells, trx1 trx2 cells were unable to
accumulate or maintain high levels of dNTPs when -factor- or
cdc15-arrested cells were allowed to reenter the cell cycle. At 80 min
after release, when the fraction of cells in S phase was maximal, the
dNTP pools in trx1 trx2 cells were 60% that of wild-type cells.
The data suggest that, in the absence of thioredoxin, cells cannot
support the high rate of dNTP synthesis required for efficient DNA
synthesis during S phase. The results constitute in vivo evidence for
thioredoxin being a physiologically relevant electron donor for
ribonucleotide reductase during DNA precursor synthesis. |
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dc.language.iso |
eng |
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dc.publisher |
J Biol Chem |
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dc.rights |
info:eu-repo/semantics/openAccess |
tr_TR |
dc.title |
Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase |
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dc.type |
article |
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dc.relation.journal |
J Biol Chem |
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dc.contributor.department |
İnönü Üniversitesi |
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dc.identifier.volume |
281 |
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dc.identifier.issue |
22 |
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dc.identifier.startpage |
15058 |
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dc.identifier.endpage |
0 |
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