dc.contributor.author |
Otlu, Barış |
|
dc.contributor.author |
Bayındır, Yaşar |
|
dc.contributor.author |
Özdemir, Fatih |
|
dc.contributor.author |
İnce, Volkan |
|
dc.contributor.author |
Çuğlan, Serpil |
|
dc.contributor.author |
Hopoğlu, Murat |
|
dc.contributor.author |
Yakupoğulları, Yusuf |
|
dc.contributor.author |
Kızılkaya, Canan |
|
dc.contributor.author |
Kuzucu, Çiğdem |
|
dc.contributor.author |
Işık, Burak |
|
dc.contributor.author |
Yılmaz, Sezai |
|
dc.date.accessioned |
2017-07-10T07:49:25Z |
|
dc.date.available |
2017-07-10T07:49:25Z |
|
dc.date.issued |
2015 |
|
dc.identifier.citation |
Otlu, B. Bayındır, Y. Özdemir, F. İnce, V. Çuğlan, S. Hopoğlu, M. Yakupoğulları, Y. Kızılkaya, C. Kuzucu, Ç. Işık, B. Yılmaz, S. (2015). Rapid Detection of Bloodstream Pathogens in Liver Transplantation Patients With FilmArray Multiplex Polymerase Chain Reaction Assays Comparison With Conventional Methods. Transplantation Proceedings, 47(6), 1926–1932. |
tr_TR |
dc.identifier.issn |
00411345 |
|
dc.identifier.uri |
http://linkinghub.elsevier.com/retrieve/pii/S0041134515005011 |
|
dc.identifier.uri |
http://hdl.handle.net/11616/7353 |
|
dc.description.abstract |
Background. Bloodstream infection (BSI) is an important concern in transplant patients.
Early intervention with appropriate antimicrobial therapy is critical to better clinical outcome;
however, there is significant delay when conventional identification methods are used.
Methods. We aimed to determine the diagnostic performance of the FilmArray BloodCulture
Identification Panel, a recently approved multiplex polymerase chain reaction assay detecting
24 BSI pathogens and 3 resistance genes, in comparison with the performances of conventional
identification methods in liver transplant (LT) patients. A total of 52 defined sepsis episodes
(signal-positive by blood culture systems) from 45 LT patients were prospectively studied.
Results. The FilmArray successfully identified 37 of 39 (94.8%) bacterial and 3 of 3
(100%) yeast pathogens in a total of 42 samples with microbial growth, failing to detect
only 2 of 39 (5.1%) bacterial pathogens that were not covered by the test panel. The
FilmArray could also detect additional pathogens in 3 samples that had been reported as
having monomicrobial growth, and it could detect Acinetobacter baumannii in 2 samples
suspected of skin flora contamination. The remaining 8 blood cultures showing a positive
signal but yielding no growth were also negative by this assay. Results of MecA, KPC, and
VanA/B gene detection were in high accordance. The FilmArray produced results with
significantly shorter turnaround times (1.33 versus 36.2, 23.6, and 19.5 h; P < .05) than
standard identification methods, Vitek II, and Vitek MS, respectively.
Conclusions. This study showed that the FilmArray appeared as a reliable alternative
diagnostic method with the potential to mitigate problems with protracted diagnosis of the
BSI pathogens in LT patients. |
tr_TR |
dc.language.iso |
eng |
tr_TR |
dc.publisher |
Transplantation Proceedings |
tr_TR |
dc.relation.isversionof |
10.1016/j.transproceed.2015.02.025 |
tr_TR |
dc.rights |
info:eu-repo/semantics/openAccess |
tr_TR |
dc.title |
Rapid detection of bloodstream pathogens in liver transplantation patients with film array multiplex polymerase chain reaction assays comparison with conventional methods |
tr_TR |
dc.type |
article |
tr_TR |
dc.relation.journal |
Transplantation Proceedings |
tr_TR |
dc.contributor.department |
İnönü Üniversitesi |
tr_TR |
dc.contributor.authorID |
101949 |
tr_TR |
dc.identifier.volume |
47 |
tr_TR |
dc.identifier.issue |
6 |
tr_TR |
dc.identifier.startpage |
1926 |
tr_TR |
dc.identifier.endpage |
1932 |
tr_TR |