Özet:
The correlation between matrix metalloproteinase
(MMP)-2, MMP-9, and MMP-14 expression on the prognostic
parameters of gallbladder carcinoma (GBC) and their role in
carcinogenesis were evaluated. Carcinomas of the gallbladder
(n = 20) and chronic cholecystitis (n= 10) were studied for the
expression of MMP-2, MMP-9, and MMP-14 by immunohistochemistry.
In all of the cases, metaplastic and dysplastic
epithelial alterations, and (in GBC histologic type, grade of
differentiation, level of infiltration, perineural and angiolymphatic
invasion, liver invasion, and lymph node involvement
were noted. MMP-2, MMP-9, MMP-14 were expressed in
tumor epithelium in 9 (45%), 20 (100%), and 20 (100%) of the
cases, respectively. MMP stromal expression including muscle
layer, vascular endothelium, fibroblasts, and lymphoid cells were
detected in all cases. MMP-2 was not expressed in normal,
metaplastic, and dysplastic epithelia. In contrast, MMP-9 and
MMP-14 immunoreactivities were present in antral-type metaplastic
areas as moderate (grade 2) and strong in dysplastic
epithelia (grade 3). Only in mucinous-type GBC was the
expression of the MMPs lower than in the other types. No
significant correlation was detected with the grade of differentiation,
level of infiltration, perineural and angiolymphatic
invasion, liver invasion, or lymph node involvement. These data
suggest that MMP-9 and MMP-14 overexpression may have an
important role in tumorigenesis. MMP-2, MMP-9, and MMP-
14 were expressed in GBC epithelium but also the expression in
the stromal component may be essential for the malignant
potential of GBC.
