Do antıbıotıcs contrıbute to postoperatıve ıleus contractıle responses of ıleum smooth muscle ın guınea pıgs to long term parenteral ceftrıaxone and ampıcıllın

Abstract

Background: Antibiotics may impair small bowel smooth muscle contractility and contribute to postoperative ileus. The aim ofthis study was to compare the contractile responses of ileum smooth muscle to different agonists in guinea pigs treated withceftriaxone (Rocephin; F. Hoffman–La Roche, Kaiseraugst, Switzerland) or ampicillin (Ampisina; Mustafa Nevzat_Ilacx SanayiiASx, Istanbul, Turkey).Methods: Twenty-four adult guinea pigs were randomly divided into three groups. Whereas eight of these received ceftriaxonesodium (100 mg/kg per day, i.m.) for 10 days, another eight guinea pigs received ampicillin (50 mg/kg per day, i.m.) for 10 days andthe remaining eight served as the control group receiving 1 mL distilled water during 10 days as placebo. By the end of 10 days, theanimals were killed and their ilea were excised. Ileum segments were placed in an organ bath; concentration–response relationship forcarbachol and histamine were obtained by adding the reagent cumulatively to the bath.Results: pD2values being the same, maximum contractile responses (Emax) to carbachol and histamine were significantly reducedin the ceftriaxone sodium group compared with the control group. No significant differences in Emaxand pD2values to carbachol andhistamine were observed between the ampicillin group and the control group.Conclusion: These data indicate that whereas ceftriaxone may impair small bowel smooth muscle contractility, ampicillin does not.There are implications for the long-term use of parenteral antibiotics in the postoperative period.