Clinical characteristics and phenotype-genotype analysis in Turkish

Abstract

Objective: Congenital hyperinsulinism (CHI) is the commonest cause of hyperinsulinaemic hypoglycaemia in the neonatal, infancy and childhood periods. Its clinical presentation, histology and underlying molecular biology are extremely heterogeneous. The aim of this study was to describe the clinical characteristics, analyse the genotype phenotype correlations and describe the treatment outcome of Turkish CHI patients.

Design and methods: A total of 35 patients with CHI were retrospectively recruited from four large paediatric endocrine centres in Turkey. Detailed clinical, biochemical and genotype information was collected.

Results: Diazoxide unresponsiveness was observed in nearly half of the patients (n= 17; 48.5%). Among diazoxide-unresponsive patients, mutations in ABCC8/KCNJ11 were identified in 16 (94%) patients. Among diazoxide-responsive patients (n=18), mutations were identified in two patients (11%). Genotype phenotype correlation revealed that mutations in ABCC8/KCNJ11 were associated with an increased birth weight and early age of presentation. Five patients had p.L1171fs (c.3512de1) ABCC8 mutations, suggestive of a founder effect. The rate of detection of a pathogenic mutation was higher in consanguineous families compared with non-consanguineous families (87.5 vs 21%; P<0.0001).

Among the diazoxide-unresponsive group, ten patients were medically managed with octreotide therapy and carbohydraterich feeds and six patients underwent subtotal pancreatectomy. There was a high incidence of developmental delay and cerebral palsy among diazoxide-unresponsive patients.

Conclusions: This is the largest study to report genotype phenotype correlations among Turkish patients with CHI. Mutations in ABCC8 and KCNJ11 are the commonest causes of CHI in Turkish patients (48.6%). There is a higher likelihood of genetic diagnosis in patients with early age of presentation, higher birth weight and from consanguineous pedigrees.

C1 [Demirbilek, Huseyin] Great Ormond St Hosp Sick Children, Dept Neonatol, London WC1N 3JH, England.

[Demirbilek, Huseyin; Arya, Ved Bhushan; Hussain, Khalid] Great Ormond St Hosp Sick Children, Dept Paediat Endocrinol, London WC1N 3JH, England.

[Demirbilek, Huseyin; Arya, Ved Bhushan; Hussain, Khalid] UCL, Inst Child Hlth, Mol Genet Unit, Dev Endocrinol Res Grp, London WC1N 1EH, England.

[Demirbilek, Huseyin; Demirel, Fatma; Guzel, Fatma; Unal, Sevim] Ankara Childrens Hematol & Oncol Training Hosp, Dept Paediat Endocrinol, Ankara, Turkey.

[Ozbek, Mehmet Nuri; Baran, Riza Taner] Children State Hosp, Dept Paediat Endocrinol, Diyarbakir, Turkey.

[Akinci, Aysehan; Kaba, Sultan] Inonu Univ, Dept Paediat Endocrinol, Malatya, Turkey.

[Dogan, Murat] Yuzuncu Yil Univ, Dept Paediat Endocrinol, Van, Turkey.

[Houghton, Jayne; Flanagan, Sarah E.; Ellard, Sian] Univ Exeter, Sch Med, Inst Biomed & Clin Sci, Exeter EX2 5DW, Devon, England.

[Tekkes, Selahattin] Dicle Univ, Dept Med Biol & Genet, Diyarbakir, Turkey.