The Effects of Nitric Oxide on Rat Stomach Injury Induced by

Abstract

Aim: Acetylsalicylic acid (ASA, aspirin), which is one of the most frequently used drugs in the world. causes severe gastric mucosal injury. Nitric oxide (NO) is synthesized from L-arginine by nitric oxide synthase (NOS). NOS can be inhibited by N omega-nitro-L-arginine methyl ester (L-NAME) and stimulated by supplementing the diet with L-arginine (L-Arg). The aim of this study was to investigate the role of NO on gastric mucosal injury induced by ASA.

Materials and Methods: Male Sprague-Dawley rats were divided into seven groups: control, ASA, ASA+L-NAME, ASA+L-Arg, ASA+L-Arg+L-NAME, only L-NAME. and only L-Arg groups. After administration of the drugs, the rats were decapitated and their stomachs were removed and fixed in 10% neutral-buffered formalin solution.

Results: Mucosal erosion, intramucosal hemorrhage, inflammatory cell infiltration, gland cell detachment, and necrosis were observed in the ASA group. It was demonstrated that L-Arg administration decreased the gastric mucosal injury, whereas L-NAME administration increased the extent and severity of the gastric injury induced by ASA. L-Arg or L-NAME administration alone did not affect gastric mucosa.

Conclusions: We concluded that NO may have protective effects on gastric mucosal injury induced by ASA.

C1 [Yagmurca, Murat] Afyon Kocatepe Univ, Fac Med, Dept Histol Embryol, Afyon, Turkey.

[Ucar, Muharrem] Inonu Univ, Fac Med, Dept Anesthesiol, Malatya, Turkey.

[Fadillioglu, Ersin] Hacettepe Univ, Fac Med, Dept Physiol, TR-06100 Ankara, Turkey.

[Erdogan, Hasan] Gaziosmanpasa Univ, Fac Med, Dept Physiol, Tokat, Turkey.

[Ozturk, Feral] Inonu Univ, Fac Med, Dept Histol Embryol, Malatya, Turkey.