In this study the activity of selenium-dependent glutathione peroxidase (Se-GSH-Px) and the levels of superoxide dismutase (SOD) and malondialdehyde (MA) of rainbow trouts exposed to various concentrations of selenium were determined. Furthermore, the hematological parameters from blood tissue were studied. Although, Se-GSH-Px activity generally increased in all groups for brain, heart and spleen tissues with respect to the control group, the only statistically significant difference (p<0.05) was found in liver tissues of trouts exposed to 4 and 6 ppm sodium selenite (SS). The SOD levels of spleen, heart, liver and brain tissues of 2 and 4 ppm SS-treated fish were also significantly higher than those of control group animals (p<0.05). The MDA values, an indicator for oxidative damage in the cell, were lower in all tissues with regard to control. Furthermore, the erythrocyte number, hemoglobin amount and hematocrit value of SS-treated rainbow trouts were significantly lower than that of control animals (p<0.05). The results show that SS has an important effect on the antioxidative defense at 2 and 4 ppm levels, whereas a 6 ppm dosage appears to be lethal for rainbow trouts.
C1 Fac Arts & Sci, Dept Biol, TR-44280 Malatya, Turkey.
Fac Arts & Sci, Dept Chem, TR-44280 Malatya, Turkey.
Fac Arts & Sci, Dept Fisheries, TR-44280 Malatya, Turkey.