Objective Adults with end-stage of chronic liver diseases have lower antibody titers after hepatitis-B vaccination. We have less amount of knowledge about the effect of non-viral cause chronic liver fibrosis on vaccination. In this study, we investigated the effect of non-viral chronic liver fibrosis on hepatitis B vaccine and the effect of tetanous toxoid co-administration at the level of humoral and cellular immune responses in an experimental model. Methods Hepatitis B vaccine was administered either alone or in combination with tetanus toxoid in thioacetamide-induced fibrotic BALB/c mice. Fibrosis level was determined by Knodell scoring. Anti-HBsAg, biochemical parameters, inflammatory (IL-1 beta, TNF-alpha), and anti-inflammatory (IL-10) cytokine levels were investigated in serum samples by automated systems and ELISA; respectively. Frequencies of activated lymphocytes were determined in flow cytometer. Results Antibody titers significantly decreased after immunization of fibrotic mice. However, co-administration of toxoid significantly elevated antibody titer. The percentage of CD19(+)CD69(+) B lymphocytes was found to be lower in vaccinated fibrotic group compared to vaccinated naive group. Simultaneous administration of toxoid significantly increased the frequencies of CD4(+) and CD8(+) T cells expressing CD69 and CD127. Interestingly, CD19(+)CD5(+)CD1(high) Breg cells were significantly reduced in the group vaccinated with hepatitis B vaccine and toxoid, simultaneously. The reduction in Breg percentage did not expose a significant decrease in the level of IL-10. Conclusion Non-viral chronic liver fibrosis causes a reduction on specific antibody level after vaccination. Reduction on Breg cell frequency may have an effect on elevation of antibody level after co-administration of tetanus toxoid.
C1 [Kayhan, Basak; Karaca, Zeynal Mete; Canpolat, Esra] Inonu Univ, Liver Transplantat Inst, Dept Gen Surg, Transplantat Immunol Lab, Malatya, Turkey.
[Kayhan, Basak] Anadolu Univ, Fac Pharm, Dept Pharmaceut Microbiol, Eskisehir, Turkey.
[Karaca, Zeynal Mete] Inonu Univ, Fac Med, Dept Med Biol & Genet, Malatya, Turkey.
[Ersan, Veysel; Yilmaz, Sezai] Inonu Univ, Liver Transplantat Inst, Dept Gen Surg, Malatya, Turkey.
[Gul, Mehmet] Inonu Univ, Fac Med, Dept Histol & Embryol, Malatya, Turkey.
[Yologlu, Saim] Inonu Univ, Fac Med, Dept Biostat & Med Informat, Malatya, Turkey.
C3 Inonu University; Anadolu University; Inonu University; Inonu
University; Inonu University; Inonu University