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Lipid peroxidation products and NO levels after adnexal torsion/detorsion in rats pretreated with aminoguanidine
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Lipid peroxidation products and NO levels after adnexal torsion/detorsion in rats pretreated with aminoguanidine
Hasçalık, Şeyma; Çelik, Önder; Türköz, Yusuf; Cığremış, Yılmaz; Mızrak, Bülent; Yoloğlu, Saim
Tarih:
2004
Özet:
Öz:Amaç: Bu deneysel çalışma rat ovarian torsion/detorsion modelinde amnioguanidin tedavisinin over dokusunda nitrik oksid (NO) ve lipid peroksidasyon ürünleri olan malondialdehid (MDA), redükte glutatyon (GSH) ve ksantin oksidaz (XO) düzeyleri üzerine olan etkisini saptamak için planlandı. Gereç ve Yöntemler: Otuziki erişkin dişi-albino rat eşit olarak 4 gruba ayrıldı (n=8): sham operasyon grubu, saline/detorsion grubu, aminoguanidine/detorsion grubu ve torsion grubu. Sham grubundaki ratlarda sağ over 360o rotasyon yapılıp hemen düzeltildi. Torsion grubundaki ratlar 360o rotasyonu takiben 3 saat sonra öldürüldü ve overleri toplandı. Aminoguanidine ve salin grubunda 3 saatlik ovarian torsion yapıldı. Bu iki grupta detorsiondan 30 dk önce intraperitoneal salin ve aminoguanidine uygulandı. Daha sonra 3 saatlik detorsionu takiben overler uzaklaştırıldı. Overlerin bir kısmı histopatolojik inceleme için formalide saklanırken bir kısmı da doku NO, MDA, GSH ve XO düzeyleri bakılmak amacıyla -30oC'de saklandı. Bulgular: Sham ve iskemi grubu ile karşılaştırıldığı zaman saline/detorsion grubunda NO düzeyleri anlamlı olarak yüksek bulundu (p<0.05). AMG grubunda ise NO düzeyleri saline grubuna göre anlamlı olarak düşük bulundu (p<0.05). İskemi/reperfüzyon grubunda MDA ve XO düzeyleri sham grubuna göre anlamlı olarak yüksek bulundu (p<0.05). Aminoguanidine tedavisi MDA ve XO düzeylerini anlamlı olarak düşürdü (p<0.05). Sham grubu ile kıyaslandığı zaman, iskemi/reperfüzyon grubunda GSH düzeyleri anlamlı olarak düşüktü (p<0.05). Aminoguanidine verilmesi GSH düzeylerinde anlamlı bir artışa yol açtı (p<0.05). Sonuç: AMG tedavisi rat ovarian iskemi-reperfüzyon modelinde serbest radikal süpürücü etkisi ile veya NO sentezini bloke ederek overleri hasarlanmadan korur.
Açıklama:
Öz (İngilizce):Objective: This experimental study was designed to determine the changes in ovarian tissue nitric oxide (NO), malondialdehyde (MDA), reduced glutathion (GSH) and xanthine oxidase (XO) levels and the effect of aminoguanidine (AMG) on these parameters after ovarian torsion/ detorsion in rats. Material and Methods: Thirty-two adult female albino rats were divided into four groups (n=8): sham operation, saline/detorsion, AMG/detorsion and torsion. The control rat underwent a sham operation wherein the right ovary was rotated 360° and then immediately relieved. Rats in the torsion group were killed after 360º clockwise adnexal torsion for 3 h. Aminoguanidine was injected intraperitoneally 30 min before detorsion in the AMG/detorsion group and saline was administered in the saline/detorsion group. After 3 h of adnexal detorsion in both of these groups, the rats were killed and adnexa was removed. A part of the adnexa was preserved in formalin with the remainder stored at -30°C until analysis to determine the tissue levels of NO, MDA, GSH and XO. Results: NO levels in saline/detorsion group were increased significantly when compared to sham operation and ischemia groups (p<0.05). NO levels in the AMG group were lower than saline/detorsion group, and differences between the two groups were statistically significant (p<0.05). Malondialdehyde and XO levels in saline/detorsion group were increased significantly when compared to sham operation group (p<0.05). MDA and XO levels in the aminoguanidine group were lower than saline/detosion group and differences between the two groups were statistically significant (p<0.05). GSH levels in the saline/detorsion group were decreased signifcantly when compared to sham operation group (p<0.05). GSH levels in the aminoguanidine group were higher than saline/detosion group, and differences between the two groups were statistically significant (p<0.05). Conclusion: This results indicates that the protective action of AMG on torsion-detorsion injury, could be attributable either by its capacity to scavenge free-radicals or its ability to inhibit iNOS.
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