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Amifostine enhances the antioxidant and hepatoprotective effects of UW and HTK preservation solutions

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dc.contributor.author Akbulut, Sami
dc.contributor.author Sevmis, Şinasi
dc.contributor.author Karakayali, Hamdi
dc.contributor.author Bayraktar, Nilufer
dc.contributor.author Unlukaplan, Muge
dc.contributor.author Öksüz, Ergun
dc.contributor.author Dagdeviren, Atilla
dc.date.accessioned 2020-07-07T09:40:34Z
dc.date.available 2020-07-07T09:40:34Z
dc.date.issued 2014-09
dc.identifier.citation Akbulut, S., Sevmis, S., Karakayali, H., Bayraktar, N., Unlukaplan, M., Oksuz, E., Dagdeviren, A. Amifostine enhances the antioxidant and hepatoprotective effects of UW and HTK preservation solutions. (2014). WORLD JOURNAL OF GASTROENTEROLOGY. tr_TR
dc.identifier.issn 1007-9327
dc.identifier.issn 2219-2840
dc.identifier.other DOI: 10.3748/wjg.v20.i34.12292
dc.identifier.uri http://abakus.inonu.edu.tr/xmlui/handle/123456789/16559
dc.description WORLD JOURNAL OF GASTROENTEROLOGY Volume: 20 Issue: 34 Pages: 12292-12300 DOI: 10.3748/wjg.v20.i34.12292 Published: SEP 14 2014 Document Type:Article tr_TR
dc.description.abstract AIM: To investigate whether amifostine contributes to the antioxidant and cytoprotective effects of histidine-tryptophan-ketoglutarate (HTK) and University of Wisconsin (UW) preservation solutions. METHODS: Forty-eight Sprague Dawley male rats were equally divided into six groups: (1) ringer Lactate (RL) group; (2) RL + amifostine (RL + A) group; (3) HTK group; (4) HTK + A group; (5) UW group; and (6) UW + A group. Rats in the RL + A, HTK + A and UW + A groups were administered amifostine intraperitoneally at a dose of 200 mg/kg prior to laparotomy. The RL group was perfused with RL into the portal vein. The RL + A group were perfused with RL into the portal vein after amifostine administration. The HTK group received an HTK perfusion while the HTK + A group received an HTK perfusion after administration of amifostine. The UW group received a perfusion of UW, while the UW + A group received a UW perfusion after amifostine administration. Liver biopsy was performed to investigate histopathological, immunochemical [transferase mediated dUTP nick end labeling (TUNEL), inducible nitric oxide syntetase (iNOS)] and ultrastructural alterations. Biochemical alterations were determined by examining levels of alanine aminotransferase, alkaline phosphatase and nitric oxide in the perfusion fluid. RESULTS: Pathological sinusoidal dilatation and centrilobular hydropic alteration were significantly lower in the groups that received amifostine prior to preservation solution perfusion. Although the best results were obtained in the UW + A group, we did not observe a statistically significant difference between the UW + A and HTK + A groups. iNOS grades were significantly lower in the amifostine groups 12 h after treatment. When the amifostine groups were compared against each other, the iNOS grades obtained from the UW + A and HTK + A groups were similar while the RL + A group had a much poorer score. TUNEL assays demonstrated a lower apoptosis ratio in the amifostine groups than in the non-amifostine groups 12 h after treatment. No statistically significant difference was observed between the UW + A and HTK + A groups for apoptosis. Cellular ultrastructure was best preserved in the UW + A and HTK + A groups. CONCLUSION: Here, we show that preoperative administration of a single dose of amifostine is sufficient to minimize the preservation damage in hepatic cells. (C) 2014 Baishideng Publishing Group Inc. All rights reserved. tr_TR
dc.language.iso en tr_TR
dc.publisher BAISHIDENG PUBLISHING GROUP INC tr_TR
dc.subject Transplantation tr_TR
dc.subject Histidine-tryptophan-ketoglutarate tr_TR
dc.subject University of Wisconsin tr_TR
dc.subject Preservation solutions tr_TR
dc.subject Antioxidant tr_TR
dc.title Amifostine enhances the antioxidant and hepatoprotective effects of UW and HTK preservation solutions tr_TR
dc.type Article tr_TR


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