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Comparison of VEGF gene silencing efficiencies of chitosan and protamine complexes containing shRNA

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dc.contributor.author Erdem-Cakmak, Fulden
dc.contributor.author Ozbas-Turan, Suna
dc.contributor.author Salva, Emine
dc.contributor.author Akbuga, Julide
dc.date.accessioned 2020-07-14T08:26:32Z
dc.date.available 2020-07-14T08:26:32Z
dc.date.issued 2014
dc.identifier.citation Erdem-Cakmak, F., Ozbas-Turan, S., Salva, E., Akbuga, J. Comparison of VEGF gene silencing efficiencies of chitosan and protamine complexes containing shRNA. (2014). CELL BIOLOGY INTERNATIONAL. tr_TR
dc.identifier.issn 1065-6995
dc.identifier.issn 1095-8355
dc.identifier.other DOI: 10.1002/cbin.10317
dc.identifier.uri http://abakus.inonu.edu.tr/xmlui/handle/123456789/16851
dc.description CELL BIOLOGY INTERNATIONAL Volume: 38 Issue: 11 Pages: 1260-1270 DOI: 10.1002/cbin.10317 Published: NOV 2014 Document Type:Article tr_TR
dc.description.abstract VEGF is an angiogenic factor promoting the proliferation and migration of endothelial cells. Inhibition of VEGF by RNAi mechanism is one of the novel and the most important strategies in antiangiogenesis therapy. In this study, the tumor silencing efficiency of ternary complexes after addition of protamine to chitosan complexes containing VEGF targeting shRNA was investigated. Besides chitosan, protamine is an effective gene delivery material. Binary and ternary complexes consisting of chitosan, protamine, and shRNA were prepared to target VEGF, their morphology, size, and zeta potential of the complexes being measured. The average size of the complexes was between 173 and 284nm and zeta potential was between +10 and 16mV. In the ternary complexes, size decreased as the chitosan ratio increased; however, its molecular weight had no effect on the size of complexes. HeLa, HEK293, and MCF-7 cell lines were used for in vitro transfection. VEGF was assayed by ELISA. A higher silencing effect was obtained using ternary complexes. Transgene expression was increased by adding protamine to chitosan complexes. Gene inhibition values in cell lines followed the rank HEK293>HeLa>MCF-7. The addition of protamine to the chitosan/shRNA (VEGF) complexes increased the knockdown of VEGF genes in the cell lines, and no cytotoxicity was found after the complexes had been incorporated into the cells. tr_TR
dc.description.sponsorship Marmara University -- SAG-D-110412-0094 tr_TR
dc.language.iso en tr_TR
dc.publisher WILEY, tr_TR
dc.subject short hairpin RNA tr_TR
dc.subject protamine tr_TR
dc.subject complex tr_TR
dc.subject VEGF tr_TR
dc.subject chitosan tr_TR
dc.title Comparison of VEGF gene silencing efficiencies of chitosan and protamine complexes containing shRNA tr_TR
dc.type Article tr_TR


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