Turkcuoglu, IlginSinaci, SelcanMelekoglu, RaufUnuvar, SongulCelik, Nesibe ZeyveliYasar, SeymaPolat, Busra Berfin2026-04-042026-04-0420251476-70581476-4954https://doi.org/10.1080/14767058.2025.2597589https://hdl.handle.net/11616/109230Objective To compare interferon-linked/inflammatory biomarkers (neopterin, indoleamine-2,3-dioxygenase [IDO], and YKL-40) in maternal serum and amniotic fluid between Trisomy-21 and chromosomally normal pregnancies. Methods This prospective case-control study included pregnancies diagnosed with Trisomy-21 and chromosomally normal pregnancies as controls. Neopterin, IDO, and YKL-40 levels in maternal serum and amniotic fluid samples were measured using ELISA. Results Neopterin and YKL-40 levels were significantly higher in amniotic fluid (p < 0.05) and maternal serum (p < 0.05), respectively, in the Trisomy-21 group. Maternal serum IDO showed a trend toward increase in Trisomy-21 pregnancies, while amniotic fluid levels did not differ between groups. All measurements were above assay detection limits. Conclusion Trisomy-21 pregnancies showed elevated AF neopterin and elevated MS YKL-40, while IDO was not significantly different. This pattern is compatible with differences in interferon-linked inflammatory/vascular pathways in Trisomy-21 pregnancies, but does not establish a mechanism. Further studies are needed to validate these findings.eninfo:eu-repo/semantics/openAccessTrisomy-21neopterinindoleamine-2,3-dioxygenaseYKL-40immune activationinflammationArticle3814136060910.1080/14767058.2025.25975892-s2.0-105024146623Q1WOS:001636114300001Q30000-0002-8342-19560000-0003-3118-40360000-0003-1300-33930000-0001-7113-66910000-0001-8454-490X0000-0002-8332-7273