Yürekli, Muhittin2020-09-092020-09-091998YÜREKLİ M (1998). The effects of phenoxybenzamine on tyrosine hydroxylase (TH) and mRNA level in adrenal medulla of sprague dawley rats. Turkish Journal of Medical Sciences, 28(1), 35 - 39.1300-01441303-6165https://hdl.handle.net/11616/17996https://search.trdizin.gov.tr/yayin/detay/6248Yıl: 1998Cilt: 28Sayı: 1ISSN: 1300-0144 / 1303-6165Sayfa Aralığı: 35 - 39Metin Dili:İngilizceAbstract: The effects of anti hipertensive phenoxybenzamine were investigated on tyrosine hydroxylase (TH) enzyme activity and TH mRNA levels. In the present study 5 months male sprague dawley (SD) rats were used. Phenoxybenzamine was injected i.p as 20 mg/kg which prepared in the 0.9 % NaCI and 5% ethanole. TH activity was measured by detecting of formation of 3H2O as a formation of dopa from 3H-tyrosine by radioisotope technique total adrenal RNA was isolated and hybridized wit 32P labeled cDNA. TH mRNA was assayed by densitometric scanning of the autoradiograms using a densitometer. TH activity and TH mRNA levels were found to be significantly increased by the effcet of phenoxybenzamine (P<0.01).trinfo:eu-repo/semantics/openAccessArticle28135396248