Cakiri, TugrulBasturk, AhmetPolat, CemalAslaner, ArifDurgut, HimmetSehirli, Ahmet OzerGul, Mehmet2024-08-042024-08-0420150102-86501678-2674https://doi.org/10.1590/S0102-865020150040000003https://hdl.handle.net/11616/96770PURPOSE: To determine the antioxidant and anti-inflammatory effects of alfa lipoic acid (ALA) on the liver injury induced by methotrexate (MTX) in rats. METHODS: Thirty two rats were randomly assigned into four equal groups; control, ALA, MTX and MTX with ALA groups. Liver injury was performed with a single dose of MTX (20 mg/kg) to groups 3 and 4. The ALA was administered intraperitonealy for five days in groups 2 and 4. The other rats received saline injection. At the sixth day the rats decapitated, blood and liver tissue samples were removed for TNF-alpha, IL-1 beta, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels measurement and histological examination. RESULTS: MTX administration caused a significant decrease in tissue GSH, and tissue Na+, K+ ATPase activity and which was accompanied with significant increases in tissue MDA and MPO activity. Moreover the pro-inflammatory cytokines (TNF-alpha, IL-beta) were significantly increased in the MTX group. On the other hand, ALA treatment reversed all these biochemical indices as well as histopathological alterations induced by MTX. CONCLUSION: Alfa lipoic acid ameliorates methotrexate induced oxidative damage of liver in rats with its anti-inflammatory and antioxidant effects.eninfo:eu-repo/semantics/openAccessThioctic AcidMethotrexateLiverRatsArticle3042472542592325710.1590/S0102-8650201500400000032-s2.0-84928747051Q3WOS:000353700300003Q4