Ulu, AhmetKoytepe, SuleymanAtes, Burhan2024-08-042024-08-0420160170-08391436-2449https://doi.org/10.1007/s00289-015-1583-1https://hdl.handle.net/11616/97082In this paper, poly (2-hydroxyethyl methacrylate) (pHEMA) and poly (2-hydroxyethyl methacrylate)-starch (pHEMA-S) composites were prepared from 2-hydroxyethyl methacrylate and different ratio starch. The pHEMA-S showed high thermal stability and biodegradability. The biodegradability of pHEMA-S composites was in the range of 14-22 % for 4 weeks depending on the starch ratio of pHEMA-S. Then, L-ASNase was immobilized onto pHEMA-S composites. The immobilized L-ASNase was investigated with optimum temperature, pH and kinetic parameters. Immobilization improved the pH stability and thermal stability of the enzyme. The K-m values were 1.58 and 0.56 mM for free and immobilized enzyme, respectively.eninfo:eu-repo/semantics/closedAccessL-asparaginasepHEMApHEMA-starch compositesImmobilizationArticle7371891190710.1007/s00289-015-1583-12-s2.0-84949518148Q2WOS:000378781000007Q3