Ozelci, DuyguYigit, Emel2024-08-042024-08-0420222619-9149https://doi.org/10.18016/ksutarimdoga.vi.865910https://search.trdizin.gov.tr/yayin/detay/1116957https://hdl.handle.net/11616/92883In this study, it was aimed to determine the most suitable protocol for the propagate of black mulberry (Morus nigra L.), one of the important gene resources of our country. by tissue culture method. For this, the most suitable explant taking time, the best concentration of 6-Benzylaminopurine (BAP) for culture initiation and the shoot propagation and the best Indole-3-Butyric Acid (IBA) concentration to achieve rooting was investigated. It was determined that. June is the right time for culture initiation, 0.75 mg L-1 BAP addition to the nutrient medium gives the best results and 1 mg L-1 BAP comes into prominence for shoot propagation. Overall, 95% rooting success was achieved in 1.5 1 mg L-1 IBA application. The period between the receipt of the explant and the completion of acclimatization lasted 20-25 weeks. It has been proved that sustainable. fast and economical propagation can be made with the developed micropropagation protocol.trinfo:eu-repo/semantics/openAccessMorus nigra L.HormoneMicropropagationTissue cultureIn vitroArticle251495610.18016/ksutarimdoga.vi.8659101116957WOS:000753046200001N/A