Gumusderelioglu, MenemseSimsek, MuratKalelioglu, DeryaCakmak, Anil SeraCoskun, SemaSana, Farzin2026-04-042026-04-0420260927-77571873-4359https://doi.org/10.1016/j.colsurfa.2025.139164https://hdl.handle.net/11616/109670Effective osseointegration and infection prevention remain critical challenges for metallic implants. In this study, we developed a facile and dual-functional coating strategy by electrospun poly(ethylene oxide) (PEO) fibers incorporated with nano-hydroxyapatite (HA) particles onto pretreated titanium (Ti) surfaces. The HA particles were synthesized via biomimetic precipitation from concentrated simulated body fluid (10 x SBF) under three different conditions: (i) at room temperature (R-HA), (ii) using microwave energy (M-HA), and (iii) in the presence of boric acid (B-HA). Crosslinking with pentaerythritol triacrylate (PETA) under UV irradiation ensured stable fiber coatings. In vitro analyses using MC3T3-E1 pre-osteoblasts and Staphylococcus epidermidis (both biofilm-forming and non-forming strains) strains revealed that while pristine PEO reduced cell attachment, HA incorporation restored proliferation and enhanced osteogenic differentiation, with B-HA showing the highest osteogenic marker expression. Meanwhile, R-HA/PEO coatings exhibited the strongest anti-adhesive effect against bacterial colonization. These results demonstrate that the combination of electrospun PEO fibers with HA-particularly boron-substituted HA-provides a synergistic approach to simultaneously promote bone integration and inhibit bacterial adhesion. This work lays the groundwork for developing smart, long-term implant coatings and highlights the potential for future in vivo studies to validate extended clinical efficacy.eninfo:eu-repo/semantics/closedAccessElectrospinningPoly (ethylene oxide)TitaniumSurface coatingImplant materialHydroxyapatiteSimulated body fluidBoronArticle73210.1016/j.colsurfa.2025.1391642-s2.0-105023959448Q1WOS:001638651600006Q20000-0001-7113-04840000-0002-6301-5184