Özbek, ETurkoz, YSahna, EOzugurlu, FMizrak, BOzbek, M2024-08-042024-08-0420001464-40961464-410Xhttps://doi.org/10.1046/j.1464-410x.2000.00531.xhttps://hdl.handle.net/11616/93252Objective To investigate the effect of melatonin on the antioxidant enzyme activity and renal tubular necrosis induced by gentamicin. Materials and methods Twenty-four adult male Sprague-Dawley rats were divided into three equal groups. In group 1, the rats were injected with vehicle (controls), in group 2 they were injected with gentamicin for 5 days and in group 3 injected with gentamicin plus melatonin for 5 days. At 24 h after the last injection, rats were killed and the renal cortex separated from the medulla. Most of the cortex was homogenized but a small sample was fixed in formaldehyde solution for histological examination by light microscopy. Blood samples were also taken to assess the serum levels of urea, creatinine, Na+, K+ and gamma-glutamyl transpeptidase (gamma-GT); before death, urine samples were analysed for protein content. Crude extracts of the cortex were used to deter-mine lipoperoxides, reduced glutathione (GSH-Px), catalase and superoxide dismutase (SOD). The results were compared using the Mann-Whitney U-test. Results Compared with the controls rats, gentamicin caused hyperproteinuria, an increase in the level gamma-GT In serum, a marked increase in lipoperoxides and a signifcant decrease of GSH-Px, catalase and SOD activity in the kidney. In the rats in group 3 there was a marked restoration in lipid peroxidation, GSH-Px, catalase, SOD activity and proteinuria, and in gamma-GT in serum. In rats in group 2 there was widespread tubular necrosis (grade 2-4) but in rats in group 3 there was a merited reduction in the extent of tubular damage. There was no significant difference in serum levels of Na+, K+, blood urea nitrogen and creatinine. Conclusion These results indicate that melatonin prevents the tubular necrosis induced by gentamicin in rats, presumably because it is a potent antioxidant and restores antioxidant enzyme activity in the rat kidney.eninfo:eu-repo/semantics/closedAccessgentamicinnephrotoxicitymelatonintubular necrosisantioxidant enzymeArticle8567427461075967710.1046/j.1464-410x.2000.00531.x2-s2.0-0034076071Q1WOS:000086446100030Q3