Subasi, BilgenGunbatan, TugbaGurbuez, IlhanInal, EbrarKartal, MuratSucu, MelikeIcen, Mehmet Sina2026-04-042026-04-0420251612-18721612-1880https://doi.org/10.1002/cbdv.202500466https://hdl.handle.net/11616/109995In this present work, in vitro lipoxygenase (LOX) inhibitory activities of methanol extracts and different fractions of six Achillea species [A. biebersteinii, A. millefolium, A. cappadocica, A. wilhelmsii, A. setacea, and A. nobilis subsp. neilreichii] were evaluated. A microplate-based spectrophotometric method was utilized for the LOX assay. The phenolic composition was analyzed using the liquid chromatography-high resolution mass spectrometer (LC-HRMS). As a result, the A. biebersteinii (Malatya locality) ethyl acetate fraction showed the highest lipoxygenase inhibitory activity (72.9% inhibition), followed by the A. cappadocica ethyl acetate fractions with 66.9% and 60.4% inhibition, respectively. The phytochemical LC-HRMS analyses revealed that the active constituents of the studied fractions were vanillic acid, hyperoside, p-coumaric acid, quercetin, and chrysoeriol. The findings showed the active bioassay-directed fractions, as well compounds to the best of our knowledge for the first time, suggesting further future biological, pharmacological, and toxicological evaluations both in vitro and in vivo.eninfo:eu-repo/semantics/closedAccessAchilleaanti-inflammatory activityhyperosidelipoxygenasevanillic acidArticle22104043496710.1002/cbdv.2025004662-s2.0-105007091950Q2WOS:001498014500001Q30000-0003-1497-30170000-0002-3670-08990000-0002-1411-71840000-0002-6022-16440000-0002-7594-51780000-0002-1524-36170000-0002-1138-3145