Akin-Polat, ZuebeydaSahin, NeslihanHkiri, ShaimaLy, Bui Minh ThuOezdemir, IsmailSemeril, David2026-04-042026-04-0420252304-6740https://doi.org/10.3390/inorganics13060204https://hdl.handle.net/11616/108573The synthesis of a series of six chloro[N-alkyl-N-cinnamyl-benzimidazol-2-yliden]silver(I) complexes was successfully achieved, wherein allyl (3a), methoxymethyl (3b), benzyl (3c), 3-fluorobenzyl (3d), 4-fluorobenzyl (3e) and 4-methyl-benzyl (3f) substituents were grafted on the benzimidazole ring. The isolated silver N-heterocyclic carbene (NHC) complexes were identified by microanalyses and mass spectrometry and characterized by FT-IR and NMR spectroscopic techniques. Conclusive evidence for the structures of complexes 3c and 3d was provided by single-crystal X-ray crystallography. The in vitro inhibitory activity of the six Ag-NHC complexes was tested against trophozoites and cysts of the pathogenic Acanthamoeba castellanii strain and the efficacy sequence is as follows: 3d > 3c > 3f > 3a > 3b > 3e. At a concentration of 100 mu M in complexes 3c, 3d and 3f and after 72 h of incubation, 5.3, 3.2 and 6.3% A. castellanii trophozoite viabilities were observed, respectively. The utilization of elevated silver(I) drug concentrations, 1000 mu M, resulted in the near-total eradication of pathogenic protozoa.eninfo:eu-repo/semantics/openAccesssilver N-heterocyclic carbene complexesspectroscopic techniquesX-ray crystal structuresAcanthamoeba castellaniiin vitro evaluationArticle13610.3390/inorganics130602042-s2.0-105009280528Q2WOS:001516151900001Q20000-0002-2582-41910000-0001-6325-0216