Yuksel, S.Tasdemir, S.Korkmaz, S.2024-08-042024-08-0420170006-92481336-0345https://doi.org/10.4149/BLL_2017_041https://hdl.handle.net/11616/97804OBJECTIVE: Protective effect of thymoquinone (TQ) against the cytotoxic and genotoxic effects of cyclophosphamide (CP) was assessed in human peripheral blood lymphocyte culture. METHODS: Mitotic indices were determined as endpoints of cytotoxicity, while sister chromatid exchanges (SCE) served as endpoints of genotoxicity. Firstly, the genotoxic effect of 0.16 mu g/ml of CP was tested and CP was detected as genotoxic. In the second set, CP group was treated with 20 mu M and 40 mu M TQ. RESULTS: TQ reduced the SCE frequencies, suggesting its protective action on human lymphocytes in vitro against the CP induced genotoxic damage. CONCLUSIONS: Our results suggest that TQ produces a protective mechanism against CP-induced genetic damage, and suggest a role of DNA strand breaks in the genotoxicity (Tab. 1, Fig. 1, Ref. 19). Text in PDF www.elis.sk.eninfo:eu-repo/semantics/openAccessthymoquinonecyclophosphamidehuman lymphocytesgenotoxicityArticle11842082112847123010.4149/BLL_2017_0412-s2.0-85018931700Q3WOS:000401303100004Q4