Atik, Ahmet EminBozdağ, Gönensin OzanAkıncı, ErsinKaya, AlaattinKoç, AhmetYalçın, TalatKarakaya, Hüseyin Çağlar2017-06-102017-06-102011Ahmet, A., Kaya, A., Akıncı, E., Yalçın, T., Koç, A., Ozan, B., Karakaya, H. Ç. (2011). Proteomic Changes During Boron Tolerance İn Barley Hordeum Vulgare And Role Of Vacuolar Proton Translocatingatpase Subunit E . Tj Bot, (135), 379–388.https://hdl.handle.net/11616/7077Boron is an essential micronutrient for plants and animals; however, it can be toxic when present at high concentrations. Th e purpose of this study was to understand the mechanisms of boron tolerance in the Turkish barley (Hordeum vulgare) Anadolu cultivar. For this purpose, 2-dimensional electrophoresis (2-DE) was used to screen diff erentially expressed proteins for both control and boron-stressed Anadolu barley genotypes. Seven proteins were revealed by 2-DE: 1) ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo large chain), 2) TLP5, a thaumatin-like protein, 3) PR5, a basic pathogenesis-related protein, 4) a RNase S-like protein, 5) a PSI type III chlorophyll a/b-binding protein, 6) a light-harvesting complex I LHC I, and 7) the vacuolar proton-translocating ATPase subunit E protein. Th ese were found to be upregulated in response to boron treatment. Even though the protein encoded by the V-ATPase subunit E gene was overexpressed, its transcript level was downregulated by boron treatment. Heterologous expression of the barley V-ATPase subunit E gene in yeast provided boron resistance to yeast cells. Th ese results indicated that the V-ATPase subunit E gene was functional and conferred tolerance to toxic boron levels in yeast and might play a role in the overall boron tolerance of barley.enginfo:eu-repo/semantics/openAccessBoron stressHordeum vulgareSaccharomyces cerevisiaeProteomicsV-ATPaseMaster Thesis379388