Emre, Fatma BilgeLopes, PaulaFerapontova, Elena E.2024-08-042024-08-0420141040-03971521-4109https://doi.org/10.1002/elan.201400048https://hdl.handle.net/11616/96472FAD entrapped into the polyethyleneimine (PEI) matrix underwent 2e(-)/2H(+) redox transformation, in contrast to the 1e(-)/1H(+) reaction observed with non-stabilized FAD-modified electrodes. Under conditions of the 2e(-)/2H(+) reaction, FAD/PEI-modified electrodes catalytically oxidized NADH starting from -100 mV vs. Ag/AgCl. These electrodes may be used for construction of NAD-dependent dehydrogenase electrodes for clinically important analytes such as glucose when coupled to the model enzyme glucose dehydrogenase. Latter allows detection of glucose starting from -100 mV within 0.05-5 mM concentration range. These results suggest the FAD stabilization by different matrices as a simple and promising approach to achieve its 2e(-)/2H(+) redox chemistry resulting in catalysis of NADH oxidation.eninfo:eu-repo/semantics/closedAccessFlavin adenine dinucleotide (FAD)NADH oxidationPolyethyleneimine (PEI)NAD-dependent glucose dehydrogenaseElectrocatalysisArticle2661354136110.1002/elan.2014000482-s2.0-84902001088Q2WOS:000337690700022Q2