Tilgen F.Er H.Mizrak B.Hepsen I.F.2024-08-042024-08-0419970146-0404https://hdl.handle.net/11616/90510Purpose. To investigate potential loxicities of mitomycin C (MMC) and 5-Fluorouracil (5-FU) histologically after administration of subconjunctivally in 8 rabbits. Methods. In group 1 (8 eyes), the right eye of 4 rabbits received a single dose of 0.2 mL subconjunctival injection of O.I. 0.2, 0.4 and 0.6 nig/ mL and an equal volume of sterile saline in the left eye. In group 2, (8 eyes) 0.2 mL subconjunctival injection of 10.0 mg/mL 5-FU and 25 mg/mL 5-FU were performed in the right eye and in the left eye once daily for 5 days, respectively. The rabbits were killed at !, 20, 30, and 45 days after the last injections, the eyes were enucleated and studied histologically using light microscopy. Results. The congestion and edema in the ciliary body and iris, polymorphonuclear leukocytes (PMNs) infiltration at the limbus, and atrophy in the extraocular muscle were prominent in eyes injected, especially, 0.4 and 0.6 mg/mL MMC than the 5-FUinjected eyes. Conclusions. Subconjunctival injection of MMC even at moderate concentrations can be toxic to the ciliary body, thus a safe dose of a single subconjunctival injection of MMC appears to be 0.1 mg/mL or less. 5-FU is also required to use of much lower total dose of subconjunctival then used in the clinica! trial.eninfo:eu-repo/semantics/closedAccess[No Keyword]Article3842-s2.0-33749211698Q1