Sare, MDemirkiran, AEAlibey, EDurmaz, B2024-08-042024-08-0420011092-6429https://doi.org/10.1089/109264201317054573https://hdl.handle.net/11616/93283Background: Perforated appendicitis can be treated laparoscopically, but this approach is associated with a higher rate of intra-abdominal abscess. Pneumoperitoneum impairs the clearance of bacteria from the peritoneal cavity in experimental models of peritonitis. The aim of this study was to investigate the effects of intra-abdominal gas insufflation on bacterial growth in a rat model. Materials and Methods: The effects of intraperitoneal insufflation with different gases and a gasless model on bacterial proliferation in a setting of Escherichia coli-induced experimental peritonitis were studied in a rat model. Saline (0.25 mL) was given intraperitoneally to six Wistar male rats as the sham group. Escherichia coli (1.5 x 10(9) cfu/mL per kilogram) was injected intraperitoneally into to 24 rats. Microorganism counts were taken after 8 hours, and rats were divided into three groups: group 1, CO2 insufflation; group 2, N2O insufflation; and group 3, no insufflation. Microorganism counts were repeated 8 hours after the procedure (at 16 hours postinjection). Results: The difference in microorganism counts between 8 and 16 hours were significant in the CO2 and N2O insufflation groups (P < 0.05) but not in the group without pneumoperitoneum. Conclusions: Abdominal insufflation may promote intra-abdominal bacterial growth or decrease intra-abdominal bacterial clearance.eninfo:eu-repo/semantics/closedAccessOpen AppendectomyPneumoperitoneumModelAppendicitisBacteremiaArticle1152852891164266410.1089/1092642013170545732-s2.0-0034788363N/AWOS:000171467100004Q2