Serarslan G.Atik E.Otlu B.Bakariş S.Durmaz R.2024-08-042024-08-0420071019-214Xhttps://hdl.handle.net/11616/90919Background and Design: This study was designed to investigate the role of proliferation markers in various benign, premalignant and malignant skin lesions and also aimed to detect HPV positivity in these lesions. Material and Method: A total of 62 paraffin blocks [12 seborrheic keratoses (SK), 10 keratoacantoma (KA), 8 actinic keratoses (AK), 22 basal cell carcinoma (BCC), and 10 squamous cell carcinoma (SCC)] were included in the study. Specimens were studied immunohistochemically for the expression of Ki-67, p21 and bcl-2. PCR was performed to detect HPV DNA. Results: HPV positivity was detected in two tissues of BCC (HPV type-16). In the lesions, the Ki-67, p21 and bcl-2 expressions were found to be increased respectively: KA<BCC<AK<SCC<SK (p<0.005), BCC<KA<SK<SCC<AK (p<0.05), AK<KA<BCC<SCC<SK (p<0.05). p21 expression was more prominent in premalignant (AK) lesions compared with malignant (BCC, SCC) ones (p<0.05). Conclusions: Our results suggest that HPV does not have a direct role in the aetiology of benign, premalignant and malignant lesions. Proliferation markers should be evaluated along with hematoxylen&eosin stained specimens for distinguishing benign, premalignant and malignant lesions. Prospective studies are still needed in this field.trinfo:eu-repo/semantics/closedAccessbcl-2Human papilloma virusKi-67p21Article41257622-s2.0-34547764337N/A