Koç, AhmetBozdağ, Gönensin O.Kaya, AlaattinKoç, AhmetNoll, Gündüla A.2017-06-062017-06-062014Bozdag Gonensin O., Kaya Alaattin, Koc Ahmet, Noll Gundula A., Prüfer Dirk, Karakaya Hüseyin Caglar (2014). Characterization of a DNA from Beta maritima that confers nickel tolerance in yeast. Gene. 2014 Apr 1;538(2):251-7.https://www.ncbi.nlm.nih.gov/pubmed/24487090https://hdl.handle.net/11616/7052Gene 538 (2014) 251–257Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.eninfo:eu-repo/semantics/openAccessNickelBeta maritimaYeastTransporter proteinLon homeostasisHeavy metal toxicityArticle000010.1016/j.gene.2014.01.052