Çağlar H.Büyüktuncel E.2024-08-042024-08-0420140975-1491https://hdl.handle.net/11616/90790Objective: This study aimed to develope a simple reversed-phase high performance chromatographic method for simultaneous determination of pseudoephdrine HCI, pheniramine maleate, acetaminophen, guaifenisin, pyrilamine maleate, chlorpheniramine maleate, triprolidine HCI, dextromethorphan HBr, diphenhydramine HCI in cough and cold pharmaceuticals.Methods: The separation of these compounds was achieved within 37.9 min on a Nucleodur gravity C18 column (250 × 4.0 mm, 5?m). The chromatographic separation of these compounds performed in a single run by using isocratic mobile phase consisting of methanol:buffer mixture (38:62, v/v) at room temperature, with flow rate of 0.75 mL.min-1. An ultraviolet absorption at 210 nm was monitored. 2,4,6-trimethoxybenzaldehyde was used as an internal standard (ISTD). The selectivity, linearity of calibration, accuracy, intraday and interday precision and forced degradation studies were examined as parts of the method validation.Results: The concentration–response relationship was linear over a concentration range of 0.2-250 ?g.mL-1for acetaminophen, 0.5–250 ?g.mL-1for pseudoephdrine HCI and pheniramine maleate, 1–250 ?g.mL-1for guaifenisin, 2.5-250 ?g.mL-1for chlorpheniramine maleate and triprolidine HCI, 5-250 ?g.mL-1for pyrilamine maleate and diphenhydramine HCI, 10-20 ?g.mL-1for dextromethorphan HBr with correlation coefficients better than 0.9993. The relative standard deviations of the intraday and interday were all less than 4%. Conclusion: The proposed liquid chromatographic method was successfully applied for the routine analysis of these compounds in different cough and cold pharmaceutical preparations such as syrups and tablets. © 2014, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved.eninfo:eu-repo/semantics/closedAccessActive ingredients cough and cold pharmaceuticalsHigh performance liquid chromatographyValidationArticle6104214282-s2.0-84908348101N/A