Karakucuk, AlptugTashan, EmineOzturk, NaileCelebi, Nevin2024-08-042024-08-0420211304-530Xhttps://doi.org/10.4274/tjps.galenos.2020.67366https://search.trdizin.gov.tr/yayin/detay/480866https://hdl.handle.net/11616/92591Objectives: The current study focused on the evaluation of the cytotoxic effect and permeability of ziprasidone hydrochloride monohydrate (ZHM) nanocrystals on Caco-2 cells. Materials and Methods: ZHM nanocrystals were prepared by the microfluidization method in the presence of polyvinylpyrrolidone as a stabilizer. Particle size (PS), particle size distribution (PDI), and zeta potential (ZP) values were measured in characterization studies. In vitro cytotoxic effects of ZHM nanocrystals were investigated using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Caco-2 transport studies were conducted with formulations of ZHM coarse powder and nanocrystals. Results: Nanocrystals were obtained with 400-600 nm PS, 0.1-0.4 PDI, and >20 mV ZP values. The cell viability remained 100% for all sample groups. The permeability value of ZHM nanocrystals through Caco-2 cells increased 2.3-fold in comparison with ZHM coarse powder. Cumulative drug transport also increased at the end of the sampling period. Conclusion: Nanocrystal technology helps to increase the permeability of drug particles by increasing the saturation solubility.eninfo:eu-repo/semantics/openAccessCaco-2 cellspermeabilityziprasidonenanocrystalArticle1822232273390226410.4274/tjps.galenos.2020.673662-s2.0-85105234645Q3480866WOS:000647198000014N/A