Ulu, AhmetNoma, Samir Abbas AliKurucay, AliTopel, Seda DemirelAsilturk, MeltemAtes, Burhan2026-04-042026-04-0420250141-81301879-0003https://doi.org/10.1016/j.ijbiomac.2025.140581https://hdl.handle.net/11616/109586In this study, we hypothesized that the emission of upconverted nanoparticles (UCNP) can trigger PEG-L-ASNase (P-Lase) activity through Forster Resonance Energy Transfer under near-infrared (NIR) irradiation. To enhance stability and activity of P-Lase, it was immobilized on functionalized NaYF4:Yb3+, Er3+, Nd3+. Upon immobilization, the obtained NaYF4:Yb3+, Er3+, Nd3+/GPTMS-P-Lase exhibited excellent pH stability, thermal stability, metal ions or organic solvent tolerance, and storage stability. The relative activity of NaYF4:Yb3+, Er3+, Nd3+/ GPTMS-P-Lase had about 65 % after 20 cycles and maintained 68 % and 59 % at +4 and 25 degrees C, respectively, after 4 weeks. Furthermore, in vitro cytotoxicity and hemolysis tests confirmed that the synthesized UCNPs were biocompatible. Most importantly, the activity of P-Lase was enhanced >= 4-fold under suitable NIR irradiation. It is reasonable to believe that this investigation may supply a novel technique to trigger the catalytic efficiency of P-Lase and may have promising application in leukemia treatment.eninfo:eu-repo/semantics/closedAccessUpconverting nanoparticlesSurface modificationL-Asparaginase immobilizationNear-infraredTriggered activityArticle3023990016310.1016/j.ijbiomac.2025.1405812-s2.0-85216788474Q1WOS:001422600900001Q1