Özeç E.Ü.İ.Taşdemİr U.Kirtay M.Esen H.H.Avunduk M.C.2024-08-042024-08-0420172146-2852https://doi.org/10.7126/CUMUDJ.345934https://hdl.handle.net/11616/91904Objectives: The aim of this study was to evaluate effect of local rifamycin application on BMP-2 expression and bone healing. Materials and Methods: A standardized 5.0-mm-diameter critical size bone defect was created mandible angulus region. In the control group (8 rats) defects were left empty. In the Group 1 (n=8 rats) defect was irrigated with rifamycin solution and 25 mg rifamycin solution injected defect area at 1, 3, 7 days after surgery. In the group 2 (n=8 rats) defects were grafted with a gelatin sponge mixed 25 mg rifamycin solution. Rats were sacrificed at 21 days after surgery. Histological slides were prepared from defect site for both immunohistochemical analysis (bone morphogenetic protein-2 (BMP-2) antibody) and histomorphometric analysis. Data were analyzed using Mann Whitney U and Kruskall Wallis test. Results: The average new bone formation, number of osteoblast and new vessel formation count were increased more in both of experimental groups in comparison with control group. Anti-BMP-2 labelling (Cell count) was increased more in both of experimental groups in comparison with control group. Conclusion: Local rifamycin application has positive effects on BMP-2 expression and bone regeneration at critical sized bone defects. © 2017. Cumhuriyet Dental Journal. All Rights Reserved.eninfo:eu-repo/semantics/openAccessBone morphogenetic protein – 2Bone regenerationCritical sized bone defectRifamycinArticle2029810510.7126/CUMUDJ.3459342-s2.0-85122996353Q4