Ceran Özcan, CananSönmez, KaanTürkyılmaz, ZaferDemiroğulları, BillurDursun, AyşeDüzgün, ErselBaşaklar, Abdullah CanKale, Nuri2017-08-162017-08-162001Ceran Özcan, C. Sönmez, K. Türkyılmaz, Z. Demiroğulları, B. Dursun, A. Düzgün, E. Başaklar, A. C. Kale, N. (2001). Effect of bilirubin in ischemia reperfusion injury on rat small intestine. Journal of Pediatric Surgery, 36(12), 1764–1767.00223468http://linkinghub.elsevier.com/retrieve/pii/S0022346801910218https://hdl.handle.net/11616/7584Purpose: The aim of this study was to determine the effects of bilirubin in experimental small intestinal ischemia/reperfusion (I/R) injury in rats. Methods: Thirty rats were divided into 5 groups (n 5 6). In group S, saline and in group B, bilirubin, 20 mg/kg were infused via the jugular vein without an additional procedure. In groups S-IR, saline, B1-IR and B2-IR, 10 and 20 mg/kg/h of bilirubin were infused for 2 hours, respectively. In these groups, an I/R procedure was done after infusions by occluding the superior mesenteric artery for 45 minutes followed by 1 hour of reperfusion. After reperfusion, the small intestines were resected for histopathologic and malondialdehyde (MDA) assessments. Mucosal lesions were scored between 0 and 5. Malondialdehyde levels and histopathologic grades were analyzed statistically. Results: Mucosal injury was severe in S-IR (grade 4 to 5), mild in B1-IR (grade 0 to 3) and none in B2-IR group (grade 0). Grades of group S-IR were higher than those of B1-IR and B2-IR statistically (P ,.05). Tissue MDA levels of the S-IR group were significantly higher than those of B1-IR and B2-IR groups (U 5 36, P , .05). Bilirubin levels correlated inversely with MDA levels (r 5 20.94). Conclusions: Bilirubin effectively prevents intestinal I/R injury in rat. This observation is consistent with the hypotheses regarding bilirubin as an antioxidant, having a role in the body defense.eninfo:eu-repo/semantics/openAccessBilirubinIschemia/reperfusion injuryIntestineArticle36121764176710.1053/jpsu.2001.28816