Browsing Temel Tıp Bilimleri by Submit Date

Now showing items 1-20 of 294

Functional characterization of new mutations in Wilson disease gene ATP7B using the yeast model

Papur, Özlenen Şimşek; Koç, Ahmet; Terzioğlu, Orhan (J Trace Elem Med Biol., 2015)

The Wilson disease gene, a copper transporting ATPase (Atp7b), is responsible for the sequestration of Cu into secretory vesicles, and this function is exhibited by the orthologous Ccc2p in the yeast. In this study, we ...
High copy overexpression screening reveals pdr5 as the main doxorubicin resistance gene in yeast

Demir, Ayşe Banu; Koç, Ahmet (PLoS One, 2015)

Doxorubicin is one of the most potent anticancer drugs used in the treatment of various cancer types. The efficacy of doxorubicin is influenced by the drug resistance mechanisms and its cytotoxicity. In this study, we ...
Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process

Muid, Khandaker Ashfaqul; Karakaya, Hüseyin Çağlar; Koç, Ahmet (Biochemical and Biophysical Research Communications, 2014)

Superoxide dismutases (SOD) serve as an important antioxidant defense mechanism in aerobic organisms, and deletion of these genes shortens the replicative life span in the budding yeast Saccharomyces cerevisiae. Even ...
Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast

Koç, Ahmet; Bozdağ, Gönensin O.; Kaya, Alaattin; Koç, Ahmet; Noll, Gündüla A. (Gene, 2014)

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima ...
Thiol peroxidase deficiency leads to ıncreased mutational load and decreased fitness in saccharomyces cerevisiae

Kaya, Alaattin; Lobanov, Alexei V.; Gerashchenko, Maxim V.; Koren, Amnon; Fomenko, Dmitri E.; Koç, Ahmet; Gladyshev, Vadim N. (Genetics, 2014)

ABSTRACT Thiol peroxidases are critical enzymes in the redox control of cellular processes that function by reducing low levels of hydroperoxides and regulating redox signaling. These proteins were also shown to regulate ...
Characterization of two genes encoding metal tolerance proteins from Beta vulgaris subspecies maritima that confers manganese tolerance in yeast

Bozdağ, Gönensin Ozan; Erbasol, Isil; Koç, Ahmet; Pedas, Pai; Karakaya, Hüseyin Çağlar (BioMetals, 2013)

Manganese (Mn2?) is an essential micronutrient in plants. However increased Mn2? levels are toxic to plant cells. Metal tolerance proteins (MTPs), member of cation diffusion facilitator protein (CDF) family, have ...
Identification of respiratory chain gene mutations that shorten replicative life span in yeast

Hacıoğlu, Elise; Demir, Ayşe Banu; Koç, Ahmet (Experimental Gerontology, 2012)

Aging is the progressive accumulation of alterations in cells that elevates the risk of death. The mitochondrial theory of aging postulates that free radicals produced by the mitochondrial respiratory system contribute ...
Proteomic changes during boron tolerance in barley Hordeum vulgare and role of vacuolar proton translocatingATPase subunit E

Atik, Ahmet Emin; Bozdağ, Gönensin Ozan; Akıncı, Ersin; Kaya, Alaattin; Koç, Ahmet; Yalçın, Talat; Karakaya, Hüseyin Çağlar (Turk J Bot, 2011)

Boron is an essential micronutrient for plants and animals; however, it can be toxic when present at high concentrations. Th e purpose of this study was to understand the mechanisms of boron tolerance in the Turkish ...
Thiol peroxidases mediate specific genome wide regulation of gene expression in response to hydrogen peroxide

Fomenko, Dima; Koç, Ahmet; Agisheva, Natalia; Michael, Jacobsen; Kaya, Alaattin; Mikalai, Malinouski; Julian, Rutherford; Siu, Kam Leung; Yan Jin, Dong; Dennis, Winge; Vadim, Gladyshe (Proceedings of the National Academy of Sciences, 2011)

Hydrogen peroxide is thought to regulate cellular processes by direct oxidation of numerous cellular proteins, whereas antioxidants, most notably thiol peroxidases, are thought to reduce peroxides and inhibit H2O2 ...
Boron stress activates the general amino acid control mechanism and ınhibits protein synthesis

Uluışık, İrem; Kaya, Alaattin; Dima, Fomenko; Karakaya, Hüseyin Çağlar; Brad, Carlson; Vadim, Gladyshev; Koç, Ahmet (PLoS ONE, 2011)

Boron is an essential micronutrient for plants, and it is beneficial for animals. However, at high concentrations boron is toxic to cells although the mechanism of this toxicity is not known. Atr1 has recently been ...
Genome wide identification of genes that play a role in boron stress response in yeast

Uluışık, İrem; Kaya, Alaattin; Avşar, Kadir; Karakaya, Hüseyin Çağlar (Genomics, 2011)

Boron is an essential micronutrient for plants and it is either necessary or beneficial for animals. Studies identified only few genes related to boron metabolism thus far and details of how boron is imported into cells and ...
Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance

Bozdağ, Gönensin Ozan; Uluışık, İrem; Gülce, Sıla Gülcüler; Karakaya, Hüseyin Çağlar; Koç, Ahmet (Biochemical and Biophysical Research Communications, 2011)

Boron is a necessary nutrient for plants and animals, however excess of it causes toxicity. Previously, Atr1 and Arabidopsis Bor1 homolog were identified as the boron efflux pump in yeast, which lower the cytosolic boron ...
The roles of thiol oxidoreductases in yeast replicative aging

Hacıoğlu, Elise; Esmer, Işıl; Fomenko, Dmitri E.; Gladyshev, Vadim N.; Koç, Ahmet (Mechanisms of Ageing and Development, 2010)

Thiol-based redox reactions are involved in the regulation of a variety of biological functions, such as protection against oxidative stress, signal transduction and protein folding. Some proteins involved in redox ...
Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes

Demir, Ayşe Banu; Koç, Ahmet (Biochemical and Biophysical Research Communications, 2010)

The free radical theory of aging states that oxidative damage to biomolecules causes aging and that antioxidants neutralize free radicals and thus decelerate aging. Mitochondria produce most of the reactive oxygen species, ...
Compartmentalization and Regulation of Mitochondrial Function by Methionine Sulfoxide Reductases in Yeast

Kaya, Alaattin; Koç, Ahmet; Lee, Byung Cheon; Fomenko, Dmitri E.; Rederstorff, Mathieu; Krol, Alain; Lescure, Alain; Gladyshev, Vadim N. (Biochemistry, 2010)

Elevated levels of reactive oxygen species can damage proteins. Sulfur-containing amino acid residues, cysteine and methionine, are particularly susceptible to such damage. Various enzymes evolved to protect proteins or ...
Effect of Thioredoxin Deletion and p53 Cysteine Replacement on Human p53 Activity in Wild type and Thioredoxin Reductase Null Yeast

Stoner, Chris; George, Perason; Koç, Ahmet; Jason, Merwin; Nathan, Lopez; Gary, Merrill (Biochemistry, 2009)

Reporter gene transactivation by human p53 is inhibited in budding yeast lacking the TRR1 gene encoding thioredoxin reductase. To investigate the role of thioredoxin in controlling p53 activity, the level of reporter ...
MsrB1 Methionine R sulfoxide Reductase 1 Knock out Mice ROLES OF MsrB1 IN REDOX REGULATION AND IDENTIFICATION OF A NOVEL SELENOPROTEIN FORM

Dima, Fomenko; Sergey, Novoselov; Sathish, Natarajan; Koç, Ahmet; Brad, Carlson; Lee, TH; Hatfield, Dolph; Vadim, Nebraska (Journal of Biological Chemistry, 2009)

Protein oxidation has been linked to accelerated aging and is a contributing factor to many diseases.Methionine residues are particularly susceptible to oxidation, but the resulting mixture of methionine R-sulfoxide ...
Identification of a novel system for boron transport: atr1 ıs a main Boron exporter in yeast_

Kaya, Alaattin; Karakaya, Hüseyin Çağlar; Fomenko, Dmitri E.; Gladyshev, Vadim N.; Koç, Ahmet (Molecular and Cellular Biology, 2009)

Boron is a micronutrient in plants and animals, but its specific roles in cellular processes are not known. To understand boron transport and functions, we screened a yeast genomic DNA library for genes that confer resistance ...
Effects of deleting mitochondrial Antioxidant genes on life span

Ünlü, Ercan Selçuk; Koç, Ahmet (Ann N Y Acad Sci., 2007)

Reactive oxygen species (ROS) damage biomolecules, accelerate aging, and shorten life span, whereas antioxidant enzymes mitigate these effects. Because mitochondria are a primary site of ROS generation and also a primary ...
Checkpoint deficient rad53 11 yeast cannot accumulate dNTPs in response to DNA damage

Koç, Ahmet; Merrill, Gary F. (Biochem Biophys Res Commun., 2007)

Deoxyribonucleotide pools are maintained at levels that support efficient and yet accurate DNA replication and repair. Rad53 is part of a protein kinase regulatory cascade that, conceptually, promotes dNTP accumulation ...

Now showing items 1-20 of 294

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