dc.contributor.author |
Özerol, İ. Halil |
|
dc.contributor.author |
Şenol, Mustafa |
|
dc.contributor.author |
Ageitos, Ana |
|
dc.contributor.author |
Talmadge, James E. |
|
dc.date.accessioned |
2015-12-07T12:51:54Z |
|
dc.date.available |
2015-12-07T12:51:54Z |
|
dc.date.issued |
1996 |
|
dc.identifier.citation |
Özerol, İ. Halil.,Şenol, M.,Ageitos, A.,Talmadge, James E.,(1996). Yüksek doz kemoterapi, otolog kemik iliği veya periferik stem cell transplantasyonu yapılacak non-Hodgkin lenfomalı hastalarda fitohemaglutinin’e cevabın araştırılması.Turgut Özal Tıp Merkezi Dergisi, 4 (4).306-310 ss. |
tr_TR |
dc.identifier.uri |
http://www.totmdergisi.org/articles/1996/volume3/issue4/1996_3_4_7.pdf |
|
dc.identifier.uri |
http://hdl.handle.net/11616/2768 |
|
dc.description |
[Journal of Turgut Özal Medical Center 1996;3(4):306-310] |
tr_TR |
dc.description.abstract |
Fitohemaglutinin (PHA), genellikle bir T lenfosit mitojenidir. CD4+ helper/inducer T lenfositlerine, CD8+ suppressor/cytotoxic T lenfositlerine göre daha fazla stimulator etki yapar. Bu çalışmada, biz normal periferik kan lökositleri (PBL) ile otolog kemik iliği (BM) ve granulosit-monosit koloni stimulan faktör (GM- CSF) ile mobilize edilmiş periferik stem cell (PSC) ürünlerinin PHA ’e verdikleri mitojenik cevapların farklı olup olmadığını araştırdık Non-Hodgkin lenfomalı 24 hastadan kemik iliği ve periferik stem cell ürünleri toplandı ve IL-2 ile 5 gün kültür yapılmadan önce ve yapıldıktan sonra PHA hücre proliferasyon cevabı incelendi. Hem PSC hem de BM hücrelerinin PHA proliferatif cevapları anlamlı şekilde normal PBL ’den düşüktü (p<0.01). PSC’in PHA cevabı BM’unkinden daha yüksekti (p<0.05). IL-2 ile kültür yapıldıktan sonra PBL’nin PHA cevabı azaldı (p<0.05) fakat PSC ve BM’unki değişmedi. Bu çalışma sonucunda IL-2 ile inkübasyona PSC ürünleri PHA'e daha yüksek mitojenik cevap verdi. Bu sonuçlara göre non-Hodgkin lenfomalı hastaların lenfositlerindeki deprese mitojenik cevap, membrana bağlı IL-2 ’nin sürekli stimüle olması nedeniyle yüksek baz proliferasyona sebep olduğunu düşündürmektedir. |
tr_TR |
dc.description.abstract |
Phytohemagglutinin (PHA) is principally a T lymphocyte mitogen, producing a greater stimulatory effect on CD4+ helper/inducer T lymphocytes than on CD8+ suppressor/cytotoxic T cells. In this study, we investigated if there are differences in the mitogenic response to PHA between autologous bone marrow (BM) and granulocyte-monocyte colony stimulating factor (GM-CSF)-mobilized peripheral stem cell (PSC) products as well as to normal peripheral blood leukocytes (PBL). BM and PSC products from 24 patients with non-Hodgkin's lymphoma (NHL) were collected and assays of cell proliferation in response to PHA were performed both before and following 5 days co-incubation with IL-2. The proliferative response to PHA by cells from both PSC and BM products were significantly lower than normal PBL (p<0.001). The PHA response of PSC was significantly higher than that observed with BM (p<0.01). The response to PHA of PSC was higher than BM (p<0.05). When compared before culture with IL-2, the PHA response of PBL was decreased (p<0.05), but PSC and BM was not changed. This study showed that the PSC products prior to IL-2 incubation had higher mitogenic response to PHA. According to these results the depressed mitogenic response of lymphocytes from non-Hodgkin patients was associated with an increase in “baseline ” proliferation due to membrane bound IL-2 resulting in continued proliferation. |
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dc.language.iso |
eng |
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dc.publisher |
İnönü Üniversitesi Tıp Fakültesi Dergisi |
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dc.rights |
info:eu-repo/semantics/openAccess |
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dc.subject |
Non-Hodgkin lenfoma |
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dc.subject |
PHA cevabı |
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dc.subject |
kemik iliği |
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dc.subject |
Periferik stem cell |
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dc.subject |
Transplantasyon |
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dc.title |
Yüksek doz kemoterapi, otolog kemik iliği veya periferik stem cell transplantasyonu yapılacak non-Hodgkin lenfomalı hastalarda fitohemaglutinin’e cevabın araştırılması |
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dc.title.alternative |
The ınvestigation of the responses to phytohemagglutinin in the patients of non-hodgkin’s lymphoma who were candidates for high dose chemotherapy, autologous bone marrow, or peripheral stem cell transplantation |
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dc.type |
article |
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dc.relation.journal |
Turgut Özal Tıp Merkezi Dergisi |
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dc.identifier.volume |
4 |
tr_TR |
dc.identifier.issue |
4 |
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dc.identifier.startpage |
306 |
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dc.identifier.endpage |
310 |
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