Özet:
Background: Primary brain tumors constitute a small percent of all malignant cancers, but their etiology
remains poorly understood. β3 integrin (ITGB3) has been recognized to play influential roles in angiogenesis,
tumor growth and metastasis. Intercellular adhesion molecule-1 (ICAM-1) is a surface glycoprotein important for
tumor invasion and angiogenesis. The aim of this study was to investigate whether specific genetic polymorphisms
of ICAM-1 and ITGB3 could be associated with brain cancer development and progression in a Turkish
population. Our study is the first to our knowledge to investigate the relationship between brain tumor risk
and ICAM-1 and β3 integrin gene polymorphisms. Materials and Methods: The study covered 92 patients with
primary brain tumors and 92 age-matched healthy control subjects. Evaluation of β3 integrin (Leu33Pro (rs5918))
and ICAM-1 (R241G (rs1799969) and K469E (rs5498)) gene polymorphisms was performed by polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: According to results of our
research, the A allele of the ICAM-1 R241G gene polymorphism appeared to be a risk factor for primary brain
tumors (p<0.001). Similarly, the frequency of the A mutant allele of ICAM-1 R241G was statistically significant
in patients with brain tumors classified as glioma (p<0.001). When allele and genotype distributions of ICAM-
1 K469E, ICAM-1 R241G and β3 integrin Leu33Pro gene polymorphisms were evaluated with age, sex, and
smoking, there were no statistically significant differences. Haplotype analysis revealed that the frequencies of
GAC (rs1799969-rs5498-rs5918) and GAT (rs1799969-rs5498-rs5918) haplotypes were significantly lower in
patients as compared with controls (p=0.001; p=0.036 respectively). Conclusions: This study provides the first
evidence that ICAM-1 R241G SNP significantly contributes to the risk of primary brain tumors in a Turkish
population. In addition, our results suggest that ICAM-1 R241G in combination ICAM-1 K469E may have
protective effects against the development of brain cancer.
