Abstract:
Caffeic acid phenethyl ester (CAPE), an active component of propolis, exhibits
antioxidant properties. This experimental study was designed to determine the
effect of CAPE on ototoxicity induced with cisplatin. Twenty-four adult Wistar albino
rats were divided into four groups: cisplatin (n=6), saline (n=6), CAPE (n=6),
and cisplatin plus CAPE (n=6). Rats were tested before and 5 days after cisplatin
treatment with or without chemo protection. The Distortion Product Otoacoustic
Emissions (DPOAEs) were elicited from the control and experimental animals utilizing
the standard commercial Otoacoustic Emission (OAEs) apparatus. The animals
in all groups were sacrificed under general anesthesia on the fifth day following last
OAE measurements. For biochemical investigations, the blood samples were drawn
from inferior vena cava
On day 0, the initial baseline DPOAEs measurement results presented similar
values while comparing the groups in drug free phase (p>0.05). On day 5, intrasubject
measurement parameters of DPgrams and I/O functions of cisplatin group
were significantly deteriorated (p<0.05). The second measurements of the other
groups revealed no significant differences between their DPgrams and I/O functions
in all frequencies (p>0.05). Among the biochemical parameters, plasma xanthine
oxidase (XO) activity was found to be more elevated in the cisplatin group than the
saline group (p<0.05). CAPE led to more decreased XO activity than cisplatin
(p<0.05). The results of this study show that prophylactic administration of CAPE
for cisplatin ototoxicity ameliorated hearing deterioration in rats.
