Abstract:
Resveratrol is as an antioxidant with free radical-scavenging activity and finds its clinical
application in the prevention of postischemic tissue injury following solid organ transplantation.
This study investigates the effect of Resveratrol on spleen and ileum tissues
subjected to hepatic ischemia-reperfusion (I/R) in rats. Twenty-four rats were recruited in
the study as follows: group A: I/R (n 8), group B: I/R Resveratrol (n 8), and group
C: sham operation (n 8). After intraperitonealy pretreatment of eight rats with
resveratrol (15 mg/kg/d) for 5 days, 16 rats were subjected to 45 minutes of hepatic
ischemia followed by 30 minutes reperfusion period. Resveratrol was given 15 minutes
prior to ischemia and just before the reperfusion in rats. After reperfusion period all rats
were sacrified. Spleen and ileum tissues were examined spectrophotometrically to measure
malondialdehyde (MDA), glutathione (GSH), and total nitrite, nitrate as an end product
of nitric oxide (NO) levels. Concerning the spleen, statistically significant decrease of GSH
and increase of MDA and NO levels were found group A when compared to groups B and
C (P .040, P .004, and P .001 group A vs group B; P .05, P .003, and P .001
group A vs group C, respectively). Parallel results were obtained in ileum. A statistically
significant decrease in GSH and an increase in MDA and NO levels in group A in respect
to group B and group C was obtained (P .048, P .034, and P .001 group A vs group
B; P .004, P .001, and P .003 group A vs group C, respectively). The result of this
study shows that resveratrol has a protective effect on spleen and ileal mitochondrial
oxidative stress in rats subjected to I/R.
