Yazar "Ünver, Tuba" seçeneğine göre listele
Listeleniyor 1 - 4 / 4
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Antifungal activity of microbial chondroitin sulfate against Candida albicans(2020) Ünver, Tuba; Erenler, Ayşe Şebnem; Melekoğlu, Rauf; Akpolat, Nusret; Seçkin, Turgay; Azimov, Raul; Özaslan, Fatma BaharAbstract: A vaginal yeast infection called vaginal candidiasis is the most commonly seen type of Candida infections with a rate of 37%. Vaginal candidiasis affects three fourth (75%) of women in their lifetimes, and this is the most common cause of women seeking gynecological care. Vaginal candidiasis can result in serious symptoms such as discharge, itching, burning sensation, pain, rash and irritation in the vagina and vulva. Candida albicans, in particular, is the most common cause of vaginal candidiasis. Therefore, the treatment and control process of Candida species is very important in terms of health. In this study, as a first step, Microbial Chondroitin Sulfate was produced from Escherichia coli C2987 strain by using a specific microbial system and reliable biotechnological methods. After that, the efficacy of Microbial CS as an antifungal agent on C. albicans strains was tested by using the agar dilution method. As a result, it was determined that the Microbial CS was particularly effective against the yeasts C. albicans, and the minimum inhibitory concentration of Microbial CS was 0.32 g/mL. Our findings showed that Microbial CS can be used in the content of various biomedical products that can be used as an antifungal agent for Candida sp. Microbial CS can also be used to treat the disease alone or to support the treatment that increases the efficacy of antibiotics.Öğe Detection of bacterial co-infection in patients with severe influenza(2023) Ünver, TubaViral infections engender bacterial colonization in the host by causing alteration in the innate immune response to common bacterial pathogens. These alterations may lead to severe bacterial pneumonia during influenza. Bacterial co-infection is common in influenza pneumonia and increases morbidity and mortality. With the onset of the COVID-19 pandemic worldwide, research in viral diseases has intensified. With this study, it can be thought that bacterial colonization may also support viral activity in the human body. This study sought to investigate bacterial co-infection in respiratory specimens using samples from the Australian influenza epidemic of 2009 as a model for analysis. Furthermore, it was thought that this study could shed light on bacterial co-infection studies in patients with COVID-19, which caused the current pandemic. 200 clinical specimens were extracted for isolation of genomic DNA and directly tested in a multiplexed quantitative PCR. Multiplex-tandem PCR (MT-PCR) assay based on 16S rRNA consensus sequence of a family (Enterobacteriaceae species) and four genera (Staphylococcus spp., Streptococcus spp., Enterococcus spp., and Pseudomonas spp.) was performed. According to MT-PCR results, respiratory tract samples showed a very high rate of bacterial identification (163/200-81.5%), and Enterococci were commonly found as the dominant genus identified in influenza patients. In a total of 200 putative co-infection samples, Enterococcus spp. was apparently present in 139 samples (69.5%), being the dominant signal (any other signal was ?10%) from 106 samples. Streptococcus spp. was next most commonly indicated with 137 positive results (68.5%), but was the dominant signal in 19 of 137. It was determined that bacterial co-infection was high in the samples taken from patients with severe influenza, and Enterococcus was the dominant bacteria in the upper respiratory tract of patients treated for severe pneumonia, while Streptococcus was more dominant in the lower respiratory tracts than the other species.Öğe The inhibitory effects of taxifolin, namely dihydroquercetin as a pharmaceutical agent on the growth of bacterial and fungal species(2024) Ünver, TubaAim: Taxifolin (dihydroquercetin) is a natural bioactive flavonoid with antifungal, antiviral, antibacterial, antioxidant, and anti-inflammatory properties, and is a valuable compound in the medical, pharmaceutical, and food industries. This study aims to determine the Minimum Inhibitory Concentration (MIC) of pure taxifolin (99.25%) against pathogenic and opportunistic microorganisms. Materials and Methods: The antibacterial and antifungal activities of taxifolin against two Gram-positive (E. aerogenes, S. aureus), three Gram-negative (P. aeruginosa, K. pneumonia and E. coli), and four yeast strains (C. krusei, C. glabrata, C. tropicalis and C. albicans) was determined using agar and broth microdilution methods. Results: The MIC value for tested Gram-positive bacteria is 1 mg/mL, which is 2 mg/ml for Gram-negative bacteria. At the same time, the MIC value was 8 mg/mL for all Candida species tested except C. glabrata (MIC: 4 mg/mL). Conclusion: In conclusion, it was determined that the antibacterial property of taxifolin was more pronounced than its antifungal properties. Taxifolin is a natural compound with high medical and pharmacological value. This study proved the effectiveness of pure taxifolin as a pharmacotherapeutic agent on pathogenic and opportunistic microorganisms. The preliminary data collected in this study should be further supported, and in vivo and bioavailability studies should be detailed in future studies.Öğe Mikrobiyal kondroitin sülfatın antifungal etkisinin çeşitli Candida suşlarında farklı antimikrobiyal test yöntemleri kullanılarak karşılaştırılması(İnönü Üniversitesi, 2022) Ünver, Tuba; Erenler, Ayşe ŞebnemKondroitin sülfat (KS), hücre yüzeyinin ve hücrelerarası matriksin önemli bir bileşenidir. N-asetilgalaktozamin ve glukuronik asitin tekrarlayan disakkarit ünitelerinden oluşan bir glikozaminoglikandır (GAG)'dır. Tıp, veterinerlik, eczacılık ve kozmetik gibi alanlarda yaygın kullanıma sahiptir. Tezimizde, biyoteknolojik yöntemler kullanılarak rekombinant E. coli (C2987) suşundan ürettiğimiz Mikrobiyal KS'nin, mantar enfeksiyonlarının tedavisinde antifungal ajan olarak kullanılabilirliği araştırılmıştır. Bu amaçla,yaygın enfeksiyon türlerinden, Candida albicans, Candida tropicalis, Candida glabrata, Candida parapsilosis ve Candida krusei çalışılmış, her tür için Minimum İnhibitör Konsantrasyon (MİK) değerleri belirlenmiştir. Sonuç olarak, Mikrobiyal KS'nin çoğu mantar türü üzerinde belirgin antifungal etkinliği olduğu kanıtlanmıştır. Vajinal flora, ?%75'ini Lactobacillus, ?%25'ini de diğer mikroorganizmaların oluşturduğu 250'den fazla mikroorganizmanın bulunduğu karmaşık bir mikrobiotadır. Buradaki doğal ortamın çeşitli nedenlerle bozulması vajinal dokudaki GAG'ların da yapısını bozarak enfeksiyon etkeni patojenlere karşı savunmasız hale getirmektedir. Mikrobiyal KS biyouyumlu, toksik olmayan ve antialerjik özellikleri nedeni ile ve polianyonik bir polimer olarak önemli bir antifungal ajan potansiyeli taşımaktadır. Çalışmamızın sonucunda antifungal aktivitesi belirlenen Mikrobiyal KS hem vajinal bağ dokudaki GAG yapısını destekleyecek hem de burada oluşabilecek muhtemel Candidal enfeksiyonlarının önüne geçebilecektir. Uyguladığımız antifungal testlerin sonuçları, Mikrobiyal KS'nin inhibitor etkisinin C. albicans ve C. parapsilosis ve C. krusei'ye karşı daha kuvvetli olduğunu göstermiştir. Bunu C. tropicalis takip etmektedir. Sonuç olarak, ürettiğimiz Mikrobiyal KS vajinal bağ dokudaki GAG yapısını destekleyecek ve oluşabilecek Candidal enfeksiyonların önüne geçebilecek potansiyeli taşımaktadır.
