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  • Küçük Resim Yok
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    Cytotoxic and genotoxic effects of nateglinide on human ovarian, prostate, and colon cancer cell lines
    (2023) Öz, Samet; Şekerci, Güldeniz; Yüksel, Furkan; Tekin, Suat
    Aim: Nateglinide, an oral anti-diabetic medication used to treat type 2 diabetes, activates ATP-dependent potassium channels in pancreatic beta cells and induces insulin secretion. Numerous antidiabetic medicines, particularly metformin, are known to drastically reduce the viability of cancer cells. This study examined the effects of nateglinide on the DNA and viability of human ovarian (A2780), prostate (LNCaP), and colon (Caco-2) cancer cells. Materials and Methods: Initially in the study, 1, 10, 100, and 1000 µM doses of nateglinide were administered for 24 hours to A2780, LNCaP, and Caco-2 cells. The 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was used to measure cell viability. Using Graphpad Prism 8, the inhibitory logarithmic concentration values (LogIC50) of nateglinide in A2780, LNCaP, and Caco-2 cells were computed based on the results of the MTT experiment. These doses were applied to A2780, LNCaP, and Caco-2 cells for the Comet assay. The Bonferroni-corrected Mann–Whitney U test was used to compare groups, and a value of p<0.05 was considered statistically significant. Results: In A2780 and LNCaP cell lines, only 1000 µM nateglinide concentration de creased cell viability (p<0.05), whereas in Caco-2 cells, all concentrations except 1 µM reduced cell viability (p<0.05). The Comet assay indicated that nateglinide produced DNA damage by increasing the tail lengths and tail moments of A2780, LNCaP, and Caco-2 cells (p<0.05) and reducing the head diameters (p<0.05). Conclusion: According to the findings of this study, nateglinide has cytotoxic effects on human ovarian, prostate and colon cancer cell lines and may possess anticancer properties.
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    Sıçanlara meteorin-like protein uygulamasının hipotalamus-hipofiz-tiroid aksı ve periferal uncoupling proteinler üzerindeki etkileri
    (İnönü Üniversitesi, 2021) Şekerci, Güldeniz
    Amaç: Yağ dokudan salgılanan Metrnl yakın zamanda keşfedilmiş peptid yapılı bir hormon olarak tanımlanmaktadır. Metrnl'nin beyin dokusunda varlığı belirlenmiş olup, soğuğa maruziyette ve obezitede serumdaki seviyesi artarak, beyaz yağ dokudaki UCP1 aktivasyonuna neden olmaktadır. Bu çalışma icv Metrnl uygulamasının HHT aksı ve periferal UCP'ler üzerindeki etkilerini araştırmak amacıyla yapıldı. Materyal ve Metot: Çalışmada erkek Spraque Dawley cinsi 40 adet sıçan kullanıldı. Sıçanlar; kontrol, sham 10 ve 100 nM Metrnl uygulanan grup olmak üzere 4'e ayrıldı (n=10). Kontrol grubu dışındaki sıçanlara ozmotik mini pompalar yardımıyla icv olarak 5µl/saat/14 gün (sham grubuna yBOS, uygulama gruplarına 10 ve 100 nM Metrnl) infüzyon gerçekleştirildi. Deney sonunda sıçanlar dekapite edilerek kan, hipotalamus, kas ile beyaz ve kahverengi yağ doku örnekleri toplandı. Alınan kan dokusundan ELISA yöntemi kullanılarak serum TSH, T3 ve T4 seviyeleri belirlendi. Ayrıca Western Blot yöntemi kullanılarak sıçanların hipotalamus dokusundan TRH, beyaz ve kahverengi yağ dokusundan UCP1, kas dokusundan UCP3 protein düzeyleri belirlendi. Bulgular: Icv olarak infüze edilen Metrnl'nin serum TSH, T3 ve T4 hormon seviyesini kontrol ve sham grubuna kıyasla artırdığı görüldü (p<0.05). Öte yandan Metrnl uygulamasının hipotalamusta TRH, beyaz ve kahverengi yağ dokuda UCP1, kas dokuda UCP3 protein seviyesini arttırdığı görüldü (p<0.05). Sonuç: Bu çalışmanın sonuçları Metrnl'nin HHT aksında rol alan hormonlar ile periferal UCP'leri aktive ettiğini göstermektedir. Anahtar Kelimeler: Metrnl, TSH, T3, T4, TRH, UCP1,UCP3
  • Küçük Resim Yok
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    Traditional and complementary medicine use in multiple sclerosis: A cross-sectional study
    (2022) Tekin, Çiğdem; Tecellioğlu, Mehmet; Şekerci, Güldeniz; Özcan, Cemal
    Aim: This objective of this study is to determine the TCM usage rates of the MS patients who are followed up at a Tertiary Healthcare Centre, the practices they mostly prefer, their level of satisfaction from these practices and their socio-demographic characteristics which may have an impact on their preferences Materials and Methods: This cross-sectional type study has been conducted on 107 MS patients who are followed up at a neurology clinic. The Kurtzke Expanded Disability Status Scale is used in order to assess the clinical inadequacy. The form of questionnaire conducted includes the questions asking their socio-demographic characteristics and TCM use of the patients. Results: It is found out that 29.9% of the MS patients have sought for the GETAT practice at least once, and 51.5% of them have intended to overcome the disease. The practices mostly used are cupping therapy/bloodletting, leech therapy, herbal medicines/phytotherapy, which are followed by osteopathy, ozone therapy and music treatment. 65.6% of the participants stated that they satisfied with or benefited from the TCM practices. It is found out that there is no significant difference (p>0.05) between the TCM use and the socio-demographic characteristics of the patients but a significant and inverse relationship between TCM use and their EDDS scores (p=0.010) exists. Conclusion: It is concluded that the MS patients use the TCM practices widely, stating that they benefited substantially from these practices. It is recommended that the behaviors of the patients regarding the use of these practices be examined in order to understand the efficacy of the medical treatment and management of the symptoms.