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Öğe The changes in some biochemical parameters in Zea mays cv. Martha F1 treated with atrazine(Academic Press Inc Elsevier Science, 2010) Akbulut, Gulcin Beker; Yigit, EmelIn this study, Zea mays cv. Martha F1 plant was treated with post-emergence atrazine in a concentration range of 27-226 mM. The effects of post-emergence atrazine treatments upon peroxidase, ascorbate peroxidase, and lipid peroxidation were investigated on the 1st, 5th, 10th, and 15th days following the treatment. In the treatment groups, peroxidase activity and malondialdehyde (MDA) content decreased after the 10th day, while ascorbate peroxidase activity was observed to decrease on the 15th day. (C) 2010 Elsevier Inc. All rights reserved.Öğe DETERMINATION OF THE EFFECTS OF BIOTIC STRESS CAUSED BY Puccinia Malvacearum IN Malva Sylvestris BY SOME BIOCHEMICAL AND PHYSIOLOGICAL PARAMETERS(Parlar Scientific Publications (P S P), 2021) Temel, Mehmet; Yigit, Emel; Kabaktepe, Sanli; Akbulut, Gulcin BekerIn this study, the effects of Puccinia malvacearum, an important rust fungus in the phylum Basidiomycota, on Malva sylvestris (mallow) were investigated. Changes in pigment content, dry weight, malondialdehyde (MDA), which is an important marker of lipid peroxidation, total phenolic content, peroxidase (POD) and ascorbate peroxidase (APX) enzymes were investigated in groups infected with rust fungi and those that were not. In the infected group, an increase in pigment content was observed in samples obtained in June and July. The increase in MDA content in infected groups was also considered an indicator that caused oxidative stress in M. sylvestris. In addition, a decrease in POD activity and an increase in phenolic content were observed in the infected group. APX activity was also increased in the infected groups. Additionally, changes in the dry weight content were observed in the infected groups. Our findings showed that M. sylvestris gave important physiological and biochemical responses to oxidative stress caused by P. malvacearum. It is thought that the data obtained in this study will contribute to the elucidation of resistance mechanisms in plants exposed to fungal infection.Öğe EFFECT OF SALICYLIC ACID AND SELENIUM ON ANTIOXIDANT SYSTEM OF AVENA SATIVA L. UNDER FENOXAPROP-P-ETHYL STRESS(Parlar Scientific Publications (P S P), 2016) Yigit, Emel; Akbulut, Gulcin Beker; Bayram, Dilek; Kaya, Armagan; Gok, YetkinThe present study focuses on understanding the role of different antioxidants in plant defense against oxidative stress caused by fenoxaprop-p-ethyl herbicide. Besides ameliorative effect of exogenously applied salicylic acid (SA) and selenium (Se) on fenoxaprop-p-ethyl-induced toxicity was examined in Avena sativa L. It was determined that the MDA (malondialdehyde) content in the groups to which only herbicide was applied was higher than that of the groups to which SA and Se applied together with the herbicide. In plants exposed to fenoxaprop-p-ethyl, the activities of peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR) and content of glutathione (GSH) increased in the treatment groups compared to the control groups. The antioxidant activity increased in the control and treatment groups that were pre-treated with SA and Se compared to the groups that were not pre-treated with SA and Se. While catalase activity (CAT) decreased in SA pre-treated plants, it increased in Se pre-treated plants. These results indicate that the fenoxaprop-p-ethyl induces various plant defense responses in target plants and that treatment with exogenous SA and Se can increase stress resistance by altering these responses.Öğe Effects of Melatonin on Morus nigra cv. 'Eksi Kara' Exposed to Drought Stress(Ankara Univ, Fac Agr, 2022) Ozelc, Duygu; Akbulut, Gulcin Beker; Yigit, EmelToday, drought stress threatens the world seriously. Determining the effects of some exogenous stimulators in acquiring resistance against stress will contribute to agriculture under drought stress. In this regard, we investigated the effects of melatonin (MEL) on Morus nigra cv. 'Eksi Kara' (black mulberry) in challenging drought. To reach this object, we reproduced 'Eksi Kara', which is registered in Turkey and has economic importance, in tissue culture by using the meristem culture method. Plants were then transferred in a medium containing polyethylene glycol (PEG) 8000, which causes-1.5 MPa drought stress, and 20 mu l MEL has applied. Leaf samples were taken on the 5th, 10th, and 15th days after treatments in groups of plants grown in a different medium (Control, Control+MEL, PEG and PEG+MEL). The changes in the pigment system, relative water content (RWC) and antioxidant system were evaluated comparatively between the groups to assess plants' growth and determine their roles in coping with stress. Our findings showed that RWC decreased in leaves under drought. Exogenous MEL added in MS medium had a mitigation effect on stress. The reduction was detected in the chlorophyll and carotenoid content of leaves. Moreover, MEL+PEG combination improved the chlorophyll level. It was seen that exogenous MEL application promoted the plant defence mechanism of M. nigra plants, which exposed to drought stress, by increasing the accumulation of non -enzymatic antioxidants; total glutathione (GSH), total phenolic, proline) and activities of antioxidant enzymes; catalase (CAT), superoxide dismutase (SOD), Glutathione-S-transferase (GST), glutathione reductase (GR), peroxidase (POD), ascorbate peroxidase (APX). This study also indicates that the application of MEL+PEG composition partially prevented membrane lipid peroxidation by decreasing (malondialdehyde) MDA content.Öğe THE EFFECTS OF ORGANIC SELENIUM ON SOME PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS IN HORDEUM VULGARE L. AND TRITICUM AESTIVUM L. EXPOSED TO SALT STRESS(Parlar Scientific Publications (P S P), 2012) Yigit, Emel; Akbulut, Gulcin Beker; Gok, Yetkin; Bayram, DilekIn this study, the protective role of organic selenium against salt stress on Hordeum vulgare L. and Triticum aestivum L. were determined. Seeds, treated with 0.004 M organic selenium in the pre-application, were germinated in environments exposed to 50, 100 and 200 mM salt stress (NaCl), in comparison to seeds without selenium treatment. The effects of 0.004 M organic selenium on peroxidase, ascorbate peroxidase, and lipid peroxidation were investigated in both plants. An evaluation of their germination and enzyme activities showed that organic selenium resulted in salient improvement when compared to the plants directly exposed to salt stress. The present study suggests that selenium can play a protective role during salt stress by enhancing the antioxidant defense system.Öğe THE EFFECTS OF REACTIVE BLACK 5 TEXTILE DYE ON PEROXIDASE ACTIVITY, LIPID PEROXIDATION AND TOTAL CHLOROPHYLL CONTENT OF PHASEOLUS VULGARIS L. CV. GINA(Parlar Scientific Publications (P S P), 2012) Kaya, Armagan; Yigit, Emel; Akbulut, Gulcin BekerThis study examined the effects of reactive black 5 (RB5) on peroxidase, lipid peroxidation and total chlorophyll content of Phaseolus vulgaris L. cv. Gina. The beans were exposed to 200, 260, 338, 439, 571, 743, 965 and 1255 m/L of RB5. Significant increase in peroxidase activity was identified in RB5-treated plants. The effects of RB5 on lipid peroxidation were analyzed. MDA content of RB5-treated plants was found to be higher in all application groups, except that of 439 mg/L. In addition, it was found that total chlorophyll content increased in all applications when compared to control group. Our findings indicate that RB5 causes significant changes in peroxidase activity, MDA content, and pigment system.Öğe THE EFFECTS OF REACTIVE PHTHALOCYANINE BLUE TEXTILE DYE ON PEROXIDASE ACTIVITY, LIPID PEROXIDATION AND TOTAL CHLOROPHYLL CONTENT OF PHASEOLUS VULGARIS L. CV. GINA(Parlar Scientific Publications (P S P), 2014) Kaya, Armagan; Yigit, Emel; Akbulut, Gulcin Beker; Ates, BurhanIn this study, the effects of Reactive Phthalocyanine Blue (RPB) changes on peroxidase, malondialdehyde (MDA) content and total chlorophyll content of Phaseolus vulgaris L. (bean) were examined. The beans were exposed to different concentrations of RPB between 200-1255 mg/L. In the analyzed plants, different effects were observed on peroxidase activity. When compared to the control group, a significant decrease was identified in the plants which were treated with RPB except for the 1255 mg/L application group. Besides the effects of RPB on MDA content were analyzed. MDA content was found to be lower in all application groups except for 439 mg/L. In addition, total chlorophyll content increased in all application groups except for 338 mg/L when compared to the control group. These findings indicated that RPB caused significant changes in peroxidase activity, MDA content and total chlorophyll. It was shown that the textile dye had a toxic effect on the plants.Öğe Effects of salicylic acid and organic selenium on wheat (Triticum aestivum L.) exposed to fenoxaprop-p-ethyl(Academic Press Inc Elsevier Science, 2018) Akbulut, Gulcin Beker; Yigit, Emel; Kaya, Armagan; Aktas, AydinIn this study, we evaluated effects of fenoxaprop-p-ethyl herbicide on wheat (Triticum aestivum L.). The wheat plants were grown in pots containing perlite. Fenoxaprop-p-ethyl was applied to adequately grown T. aestivum plants at determined doses (1 mM, 1.5 mM, 2.2 mM, 3.4 mM and 5.1 mM) via spraying. The effects of this herbicide on some antioxidant enzymes, lipid peroxidation, carotenoid, total chlorophyll and total phenolic were investigated on days 1, 5 and 10 following the application. Environmental pollutants cause oxidative stress, production of free radicals and changes in antioxidants. Synthesis of signal molecules such as salicylic acid (SA) is important for the stress response of plants. In addition to this, although selenium is known not to be an obligatory element for plants, low dose selenium has positive effects on the plant growth. We also investigated the effects that are elicited on parameters above mentioned by the treatment of exogenous 0.5 mM SA and 0.004 mM organic selenium (SeI) separately. Our findings reveal that SA and SeI, which are extemally applied before the germination of seeds that are exposed to herbicide stress, becomes effective in reduction of the negative effects of fenoxaprop-p-ethyl, with regard to antioxidant system, lipid peroxidation, pigment system and total phenolic. When we evaluate the study results, our findings suggest that especially SA plays a protective role on wheat against herbicide stress.