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Öğe The effect of Cirsium arvense extract on antioxidant status in quail(Taylor & Francis Ltd, 2013) Orhan, C.; Sahin, N.; Akdemir, F.; Markiewicz-Zukowska, R.; Borawska, M. H.; Isidorov, V. A.; Hayirli, A.1. The herb Creeping Thistle, Cirsium arvense (C. arvense), has been used in folk medicine due to its antioxidant and anti-inflammatory effects.2. The aim of this study was to investigate the effects of dietary C. arvense extract supplementation on performance, egg quality, nutrient digestibility and antioxidant status in quail.3. Quails (n=150) were allocated randomly to one of the three dietary treatments: basal diet and basal diet enriched with 100 and 200mgC. arvense extract per kg diet.4. Dietary enrichment with C. arvense extract altered neither performance and egg quality parameters nor nutrient digestibility. Although there were no changes in concentrations of vitamin A and E in serum, liver and egg yolk, supplemental C. arvense extract decreased MDA concentrations in serum, liver and egg yolk by 39.3, 40.5 and 51.5%, respectively, in a dose-response manner. As supplemental C. arvense extract increased to 200mg/kg, the activity of hepatic SOD, CAT and GSH-Px increased by 14.5, 17.4 and 35.5%, respectively.5. Addition of C. arvense extract up to 200mg to per kg diet enhanced antioxidant status in laying quail and their eggs, without affecting performance and other egg quality parameters.6. Future studies are needed to elucidate the mechanism behind the antioxidant effects of C. arvense extract.Öğe Effects of Alchemilla vulgaris on growth performance, carcass characteristics and some biochemical parameters of heat stressed broilers(Hellenic Veterinary Medical Soc, 2020) Koseman, A.; Akdemir, F.; Uremis, N.; Seker, I; Turkoz, Y.This study aimed to investigate the effects of Alchemilla vulgaris (A. vulgaris) on growth performance, carcass characteristics, and biochemical parameters in broilers exposed to high environmental temperature conditions. A total of 45 broilers of 21 days of age (Ross 308) were used and grouped as the control group (C), and the groups with the addition of 1% (G1), or 3% (G2) A. vulgaris to chicken diet, respectively. In this study, the body weights of heat stressed broilers were significantly different on the 35th day and onwards. Feed intake was higher in the control group. Feed conversion ratio (FCR) was better in groups G1 and G2 compared to control on the 36th and 42nd day, the FCR was better on the 21st-42nd days. The highest hot and cold carcass performance were observed in group G2 (79.72 +/- 0.93% and 78.02 +/- 0.99, respectively), and the lowest values were observed in group C (76.26 +/- 1.13% and 75.70 +/- 1.20%, respectively). A. vulgaris, reduced serum malondialdehyde (MDA) levels as numerically, and suppressed lipid peroxidation in a dose-dependent manner. It had significant effects on monocytes (MON %, MON count), platelets (PLT), and plateletcrit (PCT) parameters only. In conclusion, the deleterious effects of high environmental temperature in broilers could be partially reversed by A. vulgaris addition to the diets between days 21 and 42.Öğe Effects of Alchemilla vulgaris on haematology and antioxidant status of heat- stressed quails during the late laying period(Hellenic Veterinary Medical Soc, 2021) Koseman, A.; Akdemir, F.; Uremis, N.; Seker, I; Turkoz, Y.The effects of Alchemilla vulgaris (AV) on haematology and serum, liver, and ovarian antioxidant status of heat-stressed quail in the late laying period were observed in this study. A 2x3 factorial design was used with 0, 1 and 3% AV fed in thermoneutral (TN) and heat stress (HS) conditions. A total of 150 quails were randomly assigned to six groups. The quails were located in temperature controlled rooms. The mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and platelet distribution width (PDW) obtained in quail fed 1% AV were higher than in 3% AV under both TN and HS conditions. Comparing 3% AV to 1% AV, the concentration of MCH obtained for 1% AV was higher in HS and lower in TN conditions. Besides, quails fed for 1% AV had a lower procalcitonin (PCT) value in HS than 3% AV but this PCT value was the same in TN. The serum malondialdehyde (MDA) was lower in 1% AV than 3% AV in both HS and TN. The ovarian MDA was lower in TN than HS. In both TN and HS conditions, the ovarian MDA value was determined higher for 1% AV than for 3% AV. The liver glutathione (GSH) value was higher in 1% AV than 3% AV in both TN and HS conditions. The Total Oxidant Capacity (TOS) value was found higher for 3% AV in TN and 1% AV in HS. The serum GSH, TOS, and oxidative stress index (OSI) values were lower for 3% AV compared to 1% AV for both TN and HS conditions, whereas for MDA value this was the opposite. The ovarium MDA and TOS values were lower for 3% AV than for 1% AV in both TN and HS. Also, the liver MDA, GSH, and Total Antioxidant Capacity (TAS) values were lower for 3% AV than for 1% AV in both TN and HS conditions. Finally, dietary AV has been shown to have a partial antioxidative effect on the defense system and also has effect on red blood cell profiles and platelet counts rather than white blood cell profiles.Öğe Effects of dietary arginine silicate inositol complex on mineral status in rainbow trout (Oncorhynchus mykiss)(Wiley, 2008) Kucukbay, F. Z.; Yazlak, H.; Sahin, N.; Akdemir, F.; Orhan, C.; Juturu, V.; Sahin, K.Arginine silicate inositol complex (ASI; arginine 49.47 g kg(-1), silicon 8.2 g kg(-1), inositol 25 g kg(-1)), a novel composition that is a bioavailable source of silicon and arginine, has potential benefits for vascular and bone health. We have previously reported that bone mineral content increased and the amount of Ca, P, Mg and Mn in the excreta decreased in poultry with ASI supplementation. In the present study, the effect of ASI supplementation at various levels (0, 500, 1000 mg kg(-1) ASI) on growth, feed intake, feed conversion ratio (FCR) and concentrations of body elements, operculum bone ash and activity of serum alkaline phosphatase (ALP) in rainbow trout was evaluated. Ninety 0+ year-old rainbow trout with initial average weight of 50 +/- 3 g were randomly assigned to three treatment groups, three replicates of 10 fish each. The fish were fed either a basal diet or the basal diet supplemented with either 500 or 1000 mg of ASI. Body weight gain (P = 0.25), feed intake (P = 0.36) and feed efficiency (P = 0.42) were not signifcantly influenced by the dietary ASI supplementation. Per cent operculum bone ash (634 g kg(-1) versus 558 g kg(-1), P = 0.001) and ALP activity (112 UL-1 versus 92 UL-1, P = 0.001) linearly increased as dietary ASI supplementation increased. Increasing dietary ASI supplementation linearly increased serum and whole body Ca (P = 0.01), P (P = 0.01), Mg (P = 0.05; P = 0.001) Mn (P = 0.05; P = 0.01) and Zn (P = 0.01; P = 0.02) concentrations respectively. In conclusion, ASI supplementation to the basal diet significantly improved operculum bone ash and whole body mineral content in rainbow trout and did not impact feed consumption, weight gain or FCR.Öğe Effects of the supplemental chromium form on performance and oxidative stress in broilers exposed to heat stress(Elsevier, 2017) Sahin, N.; Hayirli, A.; Orhan, C.; Tuzcu, M.; Akdemir, F.; Komorowski, J. R.; Sahin, K.This experiment was conducted to investigate effects of the organic complex form of supplemental chromium (Cr) on performance, oxidative stress markers, and serum profile in broilers exposed to heat stress (HS). A total of 1,200 10-day-old boilers (Ross-308) was divided into one of the 6 treatments (2 environmental temperatures x 3 diets with different Cr forms). The birds were kept in temperature-controlled rooms at either 22 +/- 2A degrees C 24 h/d (thermoneutral, TN group) or 34 +/- 2A degrees C for 8 h/d, 08:00 to 17:00 h, followed by 22A degrees C for 16 h (HS group) and fed either a basal diet (C) or the basal diet supplemented with Cr (200 mu g/kg) through 1.600 mg of CrPic (12.43% Cr) and 0.788 mg of CrHis (25.22% Cr). Feed intake and body weight were recorded weekly. After cervical dislocation, liver samples were harvested to analyze Cr concentration and glucose transporter-2,4 (GLUT-2,4) expression. The breast meat also was sampled for the concentration of Cr and expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor kappa B (NF-kappa B). Data were analyzed by 2-way ANOVA. Heat stress caused depressions in feed intake (12.1%) and weight gain (21.1%) as well as elevations in feed conversion (11.2%) and abdominal fat (32.8%). It was also associated with depletion of Cr reserves and increases in serum concentrations of glucose, cholesterol, creatine, and enzymes. Exposure to HS was accompanied by suppression of the expressions of Nrf2 and GLUT-2 in muscle and GLUT-4 in the liver and amplification of the expression of NF-kappa B in muscle. Both Cr sources partially alleviated detrimental effects of HS on performance and metabolic profile. The efficacy of Cr as CrHis was more notable than Cr as CrPic, which could be attributed to higher bioavailability. In conclusion, CrHis can be added into the diet of broilers during hot seasons to overcome deteriorations in performance and wellbeing related to oxidative stress.
