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Öğe Caveolin-1 gene expression in rats model of chronic renal failure(2017) Özyazgan, Berna; Akgöz, Müslüm; Çiğremiş, YılmazAbstract: In this study, gene expression profile of caveoline and the kidney MDA and BUN and creatinine levels were investigated in experimentally renal failure case of rats. In the experimental group, rats were injected with 30 mg/kg of cyclosporin A via subcutaneous route for 28 days. In the control group, rats were injected with cremophor EL, vehicle for cyclosporin A, for 28 days. Caveolin gene analysis and MDA analysis in the kidney tissue as well as serum BUN and creatinine analysis were performed at the end of the experiment. Caveolin gene expression of experimental group was significantly reduced (P < 0.05), while the MDA level was significantly increased compared to those of control (P < 0.05). Serum BUN and creatinine levels were significantly increased in the experimental group compared to the control group (P < 0.05). In the Cyclosporin A induced chronic renal failure model, we suggest that the induction of the Cav-1 gene expression may prevent the renal tissue damageÖğe Effect of caffeic acid phenethyl ester (CAPE) on vascular endothelial growth factor a (VEGF-A) gene expression in gentamicin-induced acute renal nephrotoxicity(2018) Çiğremiş, Yılmaz; Akgöz, Müslüm; Özen, Hasan; Karaca, Zeynal MeteAbstract: Vascular endothelial growth factor-A (VEGF-A) gene expression in an experimental gentamicin-induced nephrotoxicity and ameliorative effect of caffeic acid phenethyl ester (CAPE) was investigated in rats. Animals were divided into four groups (n=8); control (C) group animals were given 10% dimethylsulfoxide (DMSO); gentamicin (G) group animals were given 100 mg/kg/day gentamicin; CAPE group animals were given 30 mg/kg/day CAPE and CAPE+G group animals were given 100 mg/kg/ day gentamicin plus 30 mg/kg/day CAPE. Serum creatinine and BUN levels significantly increased in gentamicin group as compared to the control group (p<0.05) while CAPE treatment did not significantly lower the levels of either BUN or creatinine (p>0.05). Gene expression level of VEGF-A in gentamicin group significantly decreased as compared to the control group, however, CAPE treatment did not have any increasing effect on the gene expression level. According to histopathological investigation, gentamicin treatment caused prominent degeneration in kidney tissue and CAPE treatment had only slight beneficial effect on lowering the tissue degeneration. The results showed that gentamicin decreases VEGF-A gene expression and this might be related to the tissue degeneration at cellular level. However, CAPE treatment did not have significant ameliorative effect in lowering the gentamicin induced nephrotoxicity.Öğe The Gene Expression of Antioxidant Enzymes in Streptozotocin-Induced Experimental Diabetes in Rat Liver Tissue(2015) Temel, Nuray; Çiğremiş, Yılmaz; Geçer, Fahrettin; Akgöz, MüslümAbstract: In this study, Wistar rats were experimentally induced diabetes and the gene expression profile of CuZn-SOD and CAT enzymes were investigated in the liver tissue of these rats. The rats were divided into two groups as control and the experimental diabetes group (DM). In group DM, as a single dose of 50 mg/kg streptozotocin (STZ) dissolved in 0.01 M citrate buffer (pH: 4.5) was given intraperitoneally to the rats. 0.01 M citrate buffer which is the vehicle for STZ was applied to the rats in control group. 72 hours after STZ treatment, blood samples were collected from the tail vein, and the blood sugar levels were measured. Rats with fasting blood sugar levels above 350 mg/dl were considered to be diabetic. The rats were decapitated 21 days after STZ treatment. The liver tissues were collected, and the gene expression profile of CuZn-SOD and CAT in the liver was measured using real-time PCR technique. In conclusion, it was found that a significant decrease was observed in the expression of CuZn-SOD and CAT genes in DM group when they were compared to that of control group.Öğe The possible hepatoprotective effect of apricot against acrylamide induced hepatotoxicity in rats(Turgut Özal Tıp Merkezi Dergisi, 2017) Erdemli, Mehmet Erman; Şahin, Nurhan; Türköz, Yusuf; Yılmaz, İsmet; Çınar, Kamuran; Akgöz, Müslüm; Ciğremiş, YılmazAbstract Objective: The aim of this study was to investigate the possible toxic effects of sub-chronic doses of acrylamide taken with drinking water on liver tissue and to test the preventive role of 5% organic dried apricot as diet supplement, in female Sprague Dawley rats. Materials and Methods: Forty female Spraque Dawley rats were divided into 4 equal groups as follows: control group (C) animals were fed with normal rat chow and tap water, apricot group (A) animals were fed with chow contain %5 apricot and tap water, acrylamide group (AA) animals were fed with normal rat chow and acrylamide at approximately 500 ?g/kg/day via tap water, acrylamide+apricot group (AA+A) animals were fed with chow contain 5% apricot and approximately 500 ?g/kg/day acrylamide via drinking water. The study procedure was maintained during 12 weeks experiment period. At the end of the study, samples of liver tissue were collected for biochemical, histopathological and molecular analyses. Results: In this study, comparison of acrylamide group liver tissue GSH levels and GSH-Px activities were found lower when compared to the control group (p < 0.05). There were no significant differences between the groups based on GST activity, histopathological results and GST-Pi gene expression mRNA levels (p < 0.05). Conclusion: It could be stated that acrylamide, at approximately 500 ?g/kg/day, ingested by rats were detoxified by the liver without resulting any liver tissue damage and application of acrylamide with sun-dried organic apricot did not change any significant molecular, histopathological and biochemical parameters in the liver. Keywords: Acrylamide; Apricot; Antioxidant Enzymes; GST-Pi; Liver.
